GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His)

GFRA1/GDNFR-alpha-1, a receptor for GDNF, mediates GDNF-induced autophosphorylation and activation of the RET receptor. It interacts with RET and forms a complex with disulfide-linked GDNF dimer, and interacts with SORL1. The SORL1-GFRA1 interaction results in GFRA1 internalization without degradation. GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived GFRA1/GDNFR-alpha-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His) is 401 a.a., with molecular weight of 55-65 KDa.

GFRA1, known as GDNFR-alpha-1, operates as a pivotal receptor for glial cell line-derived neurotrophic factor (GDNF). Its functional significance lies in mediating the GDNF-induced autophosphorylation and activation of the RET receptor. In this molecular interplay, two molecules of GDNFR-alpha are proposed to intricately engage with the disulfide-linked GDNF dimer, forming a complex alongside two molecules of RET. This interaction with RET underscores GFRA1's role in orchestrating signaling cascades critical for cellular responses. Moreover, GFRA1 exhibits additional complexities in its interactions, forming complexes with SORL1, either independently or alongside GDNF. Notably, the interaction between SORL1 and GFRA1 results in the internalization of GFRA1 without degradation, adding a layer of regulatory sophistication to its cellular functions.

Immobilized Mouse GNDF at 25 ng/mL (100 μL/well) can bind Mouse GFRA1/GDNFR-alpha-1 Protein. The ED₅₀ for this effect is 0.3750 μg/mL.

Mouse

HEK293

C-His

P97785-2 (D25-S425)

14585  [NCBI]

DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLTSGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRAVPFISVEHISKGNNCLDAAKACNLDDTCKKYRSAYITPCTTSMSNEVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDVACTERRRQTIVPVCSYEERERPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSNCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEDCLKFLNFFKDNTCLKNAIQAFGNGSDVTMWQPAPPVQTTTATTTTAFRIKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGSTHLCLSDNDYGKDGLAGASS

55-65 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.