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GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

GFRA1/GDNFR-alpha-1, a receptor for GDNF, mediates GDNF-induced autophosphorylation and activation of the RET receptor. It interacts with RET and forms a complex with disulfide-linked GDNF dimer, and interacts with SORL1. The SORL1-GFRA1 interaction results in GFRA1 internalization without degradation. GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived GFRA1/GDNFR-alpha-1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His)
  • GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

GFRA1/GDNFR-alpha-1, a receptor for GDNF, mediates GDNF-induced autophosphorylation and activation of the RET receptor. It interacts with RET and forms a complex with disulfide-linked GDNF dimer, and interacts with SORL1. The SORL1-GFRA1 interaction results in GFRA1 internalization without degradation. GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived GFRA1/GDNFR-alpha-1 protein, expressed by HEK293 , with C-His labeled tag.

Background

GFRA1, known as GDNFR-alpha-1, operates as a pivotal receptor for glial cell line-derived neurotrophic factor (GDNF). Its functional significance lies in mediating the GDNF-induced autophosphorylation and activation of the RET receptor. In this molecular interplay, two molecules of GDNFR-alpha are proposed to intricately engage with the disulfide-linked GDNF dimer, forming a complex alongside two molecules of RET. This interaction with RET underscores GFRA1's role in orchestrating signaling cascades critical for cellular responses. Moreover, GFRA1 exhibits additional complexities in its interactions, forming complexes with SORL1, either independently or alongside GDNF. Notably, the interaction between SORL1 and GFRA1 results in the internalization of GFRA1 without degradation, adding a layer of regulatory sophistication to its cellular functions.

Verified Bioactivity

Immobilized Mouse GNDF at 25 ng/mL (100 μL/well) can bind Mouse GFRA1/GDNFR-alpha-1 Protein. The ED50 for this effect is 0.3750 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Mouse GNDF at 25 ng/mL (100 μL/well) can bind Mouse GFRA1/GDNFR-alpha-1 Protein. The ED50 for this effect is 0.3750 μg/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    P97785-2 (D25-S425)

  • Gene ID
    14585  [NCBI]
  • Molecular Construction
    • N-term
    • GFRA1 (D25-S425)
      Accession # P97785-2
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    GFRA1; GDNFR-Alpha-1; Prev. GDNFRA; Glial Cell Line-Derived Neurotrophic Factor Receptor Alpha; RETL1; PI-Linked Cell-Surface Accessory Protein; TRNR1; GPI-Linked Anchor Protein; GDNF Family Receptor Alpha-1; Alternative Protein GFRA1; GFR-ALPHA-1; GDNF R

  • AA Sequence

    DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLTSGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRAVPFISVEHISKGNNCLDAAKACNLDDTCKKYRSAYITPCTTSMSNEVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDVACTERRRQTIVPVCSYEERERPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSNCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEDCLKFLNFFKDNTCLKNAIQAFGNGSDVTMWQPAPPVQTTTATTTTAFRIKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGSTHLCLSDNDYGKDGLAGASS

  • Molecular Weight

    Approximately 55-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Mouse GNDF at 25 ng/mL (100 μL/well) can bind Mouse GFRA1/GDNFR-alpha-1 Protein. The ED50 for this effect is 0.3750 μg/mL.

  • GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His)
  • GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His) Product Image