1. Recombinant Proteins
  2. Viral Proteins
  3. Hepatitis C Virus Proteins
  4. Hepatitis C virus E1 Proteins
  5. Envelope Glycoprotein E1 Protein, HCV (HEK293, His)

Envelope Glycoprotein E1 Protein, HCV (HEK293, His)

Cat. No.: HY-P74899
Handling Instructions

Envelope Glycoprotein E1 Protein, one of two subunits of the envelope glycoprotein found in the hepatitis C virus, is a type 1 transmembrane protein. It associates with envelope glycoprotein E2 as a noncovalent heterodimer, which mediates virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. E1/E2 heterodimer binds host apolipoproteins. And it also binds to CD81 and activates the epithelial growth factor receptor (EGFR) signaling pathway. Envelope Glycoprotein E1 Protein, HCV (HEK293, His) is the recombinant Virus-derived Envelope Glycoprotein E1 protein, expressed by HEK293 , with N-His labeled tag. The total length of Envelope Glycoprotein E1 Protein, HCV (HEK293, His) is 149 a.a., with molecular weight of ~18.7 kDa.

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Description

Envelope Glycoprotein E1 Protein, one of two subunits of the envelope glycoprotein found in the hepatitis C virus, is a type 1 transmembrane protein. It associates with envelope glycoprotein E2 as a noncovalent heterodimer, which mediates virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. E1/E2 heterodimer binds host apolipoproteins. And it also binds to CD81 and activates the epithelial growth factor receptor (EGFR) signaling pathway. Envelope Glycoprotein E1 Protein, HCV (HEK293, His) is the recombinant Virus-derived Envelope Glycoprotein E1 protein, expressed by HEK293 , with N-His labeled tag. The total length of Envelope Glycoprotein E1 Protein, HCV (HEK293, His) is 149 a.a., with molecular weight of ~18.7 kDa.

Background

Envelope Glycoprotein E1, one of two subunits of the envelope glycoprotein found in the hepatitis C virus, is a type 1 transmembrane protein with a highly glycosylated N-terminal ectodomain and a C-terminal hydrophobic anchor. Envelope Glycoprotein E1 associates with envelope glycoprotein E2 as a noncovalent heterodimer, which mediates virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. Envelope Glycoprotein E1 helps the virus attach to the membrane of the targeted cell. In other envelope virus Envelope Glycoprotein E1 has a similar role in helping the virus get into the cell. E1/E2 heterodimer is essential for HCV entry. E1/E2 heterodimer binds host apolipoproteins such as APOB and APOE thereby forming a lipo-viro-particle (LVP). Furthermore, association of APOE with LVP allows the initial virus attachment to cell surface receptors such as the heparan sulfate proteoglycans (HSPGs), syndecan-1 (SDC1), syndecan-1 (SDC2), the low-density lipoprotein receptor (LDLR) and scavenger receptor class B type I (SCARB1). And E1/E2 heterodimer binds to CD81 and activates the epithelial growth factor receptor (EGFR) signaling pathway[1][2][3][4].

Species

Virus

Source

HEK293

Tag

N-His

Accession

AAC15725 (Y192-I340)

Gene ID

/

Molecular Construction
N-term
His
HCV E1 (Y192-I340)
Accession # AAC15725
C-term
Synonyms
HCV-E1 Protein; Hepatitis C virus Envelope Glycoprotein E1 / HCV-E1 (subtype 1b, strain HC-J4) Protein
Molecular Weight

Approximately 18.7 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Envelope Glycoprotein E1 Protein, HCV (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Envelope Glycoprotein E1 Protein, HCV (HEK293, His)
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HY-P74899
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