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  5. HER2/CD340 Protein, Canine (HEK293, His)

HER2/CD340 Protein, Canine (HEK293, His)

Cat. No.: HY-P74897
COA Handling Instructions

The HER2/CD340 Protein is a member of the receptor tyrosine kinase family. HER2/CD340 Protein participates in the PI3K/Akt signaling pathway to regulate the differentiation and apoptosis of neural stem cells in the cochlear nucleus. HER2/CD340 Protein is an overexpressed oncogenic factor in bladder cancer that activates the oncogenic signaling pathway, thereby promoting tumor cell survival/proliferation. HER2/CD340 Protein, Canine (HEK293, His) is the recombinant canine-derived HER2/CD340 protein, expressed by HEK293 , with C-His labeled tag. The total length of HER2/CD340 Protein, Canine (HEK293, His) is 630 a.a., with molecular weight of 75- 95 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

The HER2/CD340 Protein is a member of the receptor tyrosine kinase family. HER2/CD340 Protein participates in the PI3K/Akt signaling pathway to regulate the differentiation and apoptosis of neural stem cells in the cochlear nucleus. HER2/CD340 Protein is an overexpressed oncogenic factor in bladder cancer that activates the oncogenic signaling pathway, thereby promoting tumor cell survival/proliferation. HER2/CD340 Protein, Canine (HEK293, His) is the recombinant canine-derived HER2/CD340 protein, expressed by HEK293 , with C-His labeled tag. The total length of HER2/CD340 Protein, Canine (HEK293, His) is 630 a.a., with molecular weight of 75- 95 kDa.

Background

The ErbB family consists of four separate plasma membrane-binding receptor tyrosine kinases. One of them is erbB-2, and the other members are erbB-1, erbB-3 (neuregulin-binding; Lack of kinase domains) and erbB-4. The receptor tyrosine-protein kinase erbB-2 is a protein normally found on cell membranes and is encoded by the ERBB2 gene. The human protein is also often referred to as HER 2 (Human epidermal growth factor receptor 2) or CD340 (differentiated cluster 340). HER2 has binding activity with Hsp90 protein, protein tyrosine kinase and ubiquitination protein. ErbB2 regulates differentiation and apoptosis of neural stem cells in the cochlear nucleus through PI3K/Akt pathway. HER2 is an oncogenic factor that is overexpressed in bladder cancer and activates the oncogenic signaling pathway, thereby promoting tumor cell survival/proliferation[1][2][3][4].

Biological Activity

Measured by its ability to block anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer cell proliferation. The ED50 this effect is 0.4594 μg/mL in the presence of 0.6 µg/mL Trastuzmab, corresponding to a specific activity is 2.177×103 units/mg.

  • Measured by its ability to block anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer cell proliferation. The ED50 this effect is 0.4594 μg/mL in the presence of 0.6 µg/mL Trastuzmab, corresponding to a specific activity is 2.177×103 units/mg.
Species

Canine

Source

HEK293

Tag

C-His

Accession

A0A8I3NJW9 (T23-T652)

Gene ID
Molecular Construction
N-term
HER2 (T23-T652)
Accession # A0A8I3NJW9
His
C-term
Synonyms
Receptor tyrosine-protein kinase erbB-2; MLN 19; CD340; ERBB2; HER2; NEU; NGL
AA Sequence

TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPANASLSFLQDIQEVQGYVLIAHSQVRQIPLQRLRIVRGTQLFEDNYALAVLDNGDPLEGGIPAPGAAPGGLRELQLRSLTEILKGGVLIQRSPQLCHQDTILWKDVFHKNNQLALTLIDTNRSRACPPCSPACKDAHCWGASSGDCQSLTRTVCAGGCARCKGPQPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTSCPYNYLSTDVGSCTLVCPLNNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFEGDPASNTAPLQPEQLRVFEALEEITGYLYISAWPDSLPNLSVFQNLRVIRGRVLHDGAYSLTLQGLGISWLGLRSLRELGSGLALIHRNARLCFVHTVPWDQLFRNPHQALLHSANRPEEECVGEGLACYPLCAHGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVKDRYCLPCHSECQPQNGSVTCFGSEADQCVACAHYKDPPFCVARCPSGVKPDLSFMPIWKFADEEGTCQPCPINCTHSCADLDEKGCPAEQRASPVT

Molecular Weight

75- 95 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

HER2/CD340 Protein, Canine (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HER2/CD340 Protein, Canine (HEK293, His)
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HY-P74897
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