HK1/Hexokinase-1 Protein, Human (His)

The HK1/hexokinase-1 protein phosphorylates hexose sugars, including D-glucose, D-glucosamine, D-fructose, D-mannose, and 2-deoxy-D-glucose, to form the corresponding hexose 6-phosphate. HK1/Hexokinase-1 Protein, Human (His) is the recombinant human-derived HK1/Hexokinase-1 protein, expressed by E. coli , with N-6*His labeled tag.

The Hexokinase-1 (HK1) protein plays a pivotal role in cellular metabolism by catalyzing the phosphorylation of various hexoses, including D-glucose, D-glucosamine, D-fructose, D-mannose, and 2-deoxy-D-glucose, to generate hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate, and 2-deoxy-D-glucose 6-phosphate, respectively). While HK1 does not phosphorylate N-acetyl-D-glucosamine, it mediates the critical initial step of glycolysis by catalyzing the phosphorylation of D-glucose to D-glucose 6-phosphate. Beyond its metabolic functions, HK1 is involved in innate immunity and inflammation, acting as a pattern recognition receptor for bacterial peptidoglycan. In the cytosol, the N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits HK1's activity, leading to its dissociation from the mitochondrial outer membrane and subsequently activating the NLRP3 inflammasome. This multifaceted role underscores HK1's significance in cellular processes beyond glycolysis, highlighting its involvement in immune response and inflammation.

Measured by its ability to phosphorylate glucose. The specific activity is 385.882 pmol/min/μg, as measured under the described conditions.

Human

E. coli

N-6*His

P19367-1 (E13-A475)

3098  [NCBI]

Partial

ELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLA

55.9 kDa

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.