HK1/Hexokinase-1 Protein, Human (His)
Based on 1 Customer Validation
The HK1/hexokinase-1 protein phosphorylates hexose sugars, including D-glucose, D-glucosamine, D-fructose, D-mannose, and 2-deoxy-D-glucose, to form the corresponding hexose 6-phosphate. HK1/Hexokinase-1 Protein, Human (His) is the recombinant human-derived HK1/Hexokinase-1 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The HK1/hexokinase-1 protein phosphorylates hexose sugars, including D-glucose, D-glucosamine, D-fructose, D-mannose, and 2-deoxy-D-glucose, to form the corresponding hexose 6-phosphate. HK1/Hexokinase-1 Protein, Human (His) is the recombinant human-derived HK1/Hexokinase-1 protein, expressed by E. coli , with N-6*His labeled tag.
Background
The Hexokinase-1 (HK1) protein plays a pivotal role in cellular metabolism by catalyzing the phosphorylation of various hexoses, including D-glucose, D-glucosamine, D-fructose, D-mannose, and 2-deoxy-D-glucose, to generate hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate, and 2-deoxy-D-glucose 6-phosphate, respectively). While HK1 does not phosphorylate N-acetyl-D-glucosamine, it mediates the critical initial step of glycolysis by catalyzing the phosphorylation of D-glucose to D-glucose 6-phosphate. Beyond its metabolic functions, HK1 is involved in innate immunity and inflammation, acting as a pattern recognition receptor for bacterial peptidoglycan. In the cytosol, the N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits HK1's activity, leading to its dissociation from the mitochondrial outer membrane and subsequently activating the NLRP3 inflammasome. This multifaceted role underscores HK1's significance in cellular processes beyond glycolysis, highlighting its involvement in immune response and inflammation.
Verified Bioactivity
Measured by its ability to phosphorylate glucose. The specific activity is 385.882 pmol/min/μg, as measured under the described conditions.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P19367-1 (E13-A475)
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Protein Length
Partial
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Synonyms
HK1; Glycolytic Enzyme; Hexokinase 1; Hexokinase IR; Brain Form Hexokinase; Hexokinase-2; Hexokinase Type I; NEDVIBA; Hexokinase-A; HK1-Tc; Hexokinase-1; CNSHA5; Hexokinase; HMSNR; HK1-Tb; RP79; HK1-Ta; NMSR; HKI; HXK1; Neuropathy, Hereditary Motor And Se
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AA Sequence
ELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLA
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Molecular Weight
Approximately 56 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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