1. Recombinant Proteins
  2. Cytokines and Growth Factors Receptor Proteins
  3. Interferon & Receptors Cytokine Receptors
  4. IFN-gamma Receptor
  5. IFN-gamma R2
  6. IFN-gamma R2 Protein, Mouse (HEK293, His)

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Mouse IFN-gamma R1 at 2 μg/mL can bind Biotinylated Recombinant Mouse IFN-gamma R2 in the presence of Recombinant Mouse IFN-gamma (HY-P7071). The ED50 for this effect is 0.7225 μg/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized Mouse IFN-gamma R1 at 2 μg/mL can bind Biotinylated Recombinant Mouse IFN-gamma R2 in the presence of Recombinant Mouse IFN-gamma (HY-P7071). The ED50 for this effect is 0.7225 μg/mL.
Species

Mouse

Source

HEK293

Tag

C-10*His

Accession

Q63953/NP_032364 (A20-V243)

Gene ID

15980

Molecular Construction
N-term
IFN-gamma R2 (A20-V243)
Accession # Q63953/NP_032364
10*His
C-term
Protein Length

Partial

Synonyms
Interferon gamma receptor 2; IFN-γ Receptor II; IFNGR2; IFNGT1
AA Sequence

ASSPDSFSQLAAPLNPRLHLYNDEQILTWEPSPSSNDPRPVVYQVEYSFIDGSWHRLLEPNCTDITETKCDLTGGGRLKLFPHPFTVFLRVRAKRGNLTSKWVGLEPFQHYENVTVGPPKNISVTPGKGSLVIHFSPPFDVFHGATFQYLVHYWEKSETQQEQVEGPFKSNSIVLGNLKPYRVYCLQTEAQLILKNKKIRPHGLLSNVSCHETTANASARLQQV

Predicted Molecular Mass
26.7 kDa
Molecular Weight

Approximately 35-48 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Purity
  • ≥ 95%, as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IFN-gamma R2 Protein, Mouse (HEK293, His)

 

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Product Name:
IFN-gamma R2 Protein, Mouse (HEK293, His)
Cat. No.:
HY-P74852A
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