1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. IGF family
  4. Insulin-like Growth Factor I (IGF-1)
  5. IGF-I/IGF-1 Protein, Human (P.pastoris)

IGF-I/IGF-1 Protein, Human (P.pastoris)

Cat. No.: HY-P73136
Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Size Stock
200 μg Ask For Quote & Lead Time

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells and the ED50 is typically 1.5-6 ng/mL.
Species

Human

Source

P. pastoris

Tag

Tag Free

Accession

P05019 (G49-A118)

Gene ID
Synonyms
Insulin-like growth factor I; IGF-I; MGF; Somatomedin-C; IBP1
Molecular Weight

Approximately 9.1 kDa

Purity

Greater than 95% as determined by SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IGF-I/IGF-1 Protein, Human (P.pastoris) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IGF-I/IGF-1 Protein, Human (P.pastoris)
Cat. No.:
HY-P73136
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