IKKβ Protein, Human (Sf9, GST)

IKKβ protein is a serine kinase that is critical in the NF-kappa-B signaling pathway and can respond to various stimuli such as cytokines or cellular stress. As part of the classical IKK complex, IKKβ activates NF-kappa-B through phosphorylation inhibitors, leading to its degradation and release of NF-kappa-B for gene transcription. IKKβ Protein, Human (Sf9, GST) is the recombinant human-derived IKKβ protein, expressed by Sf9 insect cells , with N-GST labeled tag.

IKKβ protein, a serine kinase, plays a pivotal role in the NF-kappa-B signaling pathway, activated by diverse stimuli such as inflammatory cytokines, bacterial or viral products, DNA damage, or cellular stresses. As a constituent of the canonical IKK complex, IKKβ contributes to the conventional NF-kappa-B activation pathway. It phosphorylates inhibitors of NF-kappa-B on critical serine residues, facilitating their polyubiquitination and subsequent degradation by the proteasome. This orchestrated modification unleashes free NF-kappa-B, allowing its translocation into the nucleus and activation of the transcription of numerous genes involved in immune response, growth control, or protection against apoptosis. Beyond NF-kappa-B inhibitors, IKKβ phosphorylates various signaling pathway components, including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. These phosphorylations exert a negative regulation on canonical IKKs and may prevent the excessive production of inflammatory mediators. Moreover, IKKβ targets substrates such as FOXO3, NAA10, NCOA3, BCL10, IRS1, RIPK1, and IRF5, influencing diverse cellular processes and responses.

Human

Sf9 insect cells

N-GST

O14920 (S695-S756)

3551

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.