1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Inter-Alpha-Trypsin Inhibitors (ITI)
  5. ITIH3
  6. ITIH3 Protein, Human (HEK293, His)

ITIH3 Protein, Human (HEK293, His)

Cat. No.: HY-P70407
COA Handling Instructions

The ITIH3 protein has a dual role: as a carrier of hyaluronic acid in serum and as a binding protein that facilitates interactions with various matrix proteins. These interactions regulate the localization, synthesis, and degradation of hyaluronic acid, which is critical for multiple cellular activities. ITIH3 Protein, Human (HEK293, His) is the recombinant human-derived ITIH3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ITIH3 Protein, Human (HEK293, His) is 617 a.a., with molecular weight of ~90.0 kDa.

For research use only. We do not sell to patients.

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Description

The ITIH3 protein has a dual role: as a carrier of hyaluronic acid in serum and as a binding protein that facilitates interactions with various matrix proteins. These interactions regulate the localization, synthesis, and degradation of hyaluronic acid, which is critical for multiple cellular activities. ITIH3 Protein, Human (HEK293, His) is the recombinant human-derived ITIH3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ITIH3 Protein, Human (HEK293, His) is 617 a.a., with molecular weight of ~90.0 kDa.

Background

The ITIH3 protein exhibits a versatile role, potentially acting as a carrier for hyaluronan in serum and serving as a binding protein that facilitates interactions between hyaluronan and various matrix proteins, including those present on cell surfaces in tissues. These interactions play a pivotal role in regulating the localization, synthesis, and degradation of hyaluronan, processes essential for cells undergoing diverse biological activities. Within the context of I-alpha-I plasma protease inhibitors, ITIH3 contributes to the assembly of these inhibitors, forming complexes with one or two heavy chains (HC) and the light chain bikunin. Notably, the pre-alpha-inhibitor (P-alpha-I) consists of ITIH3/HC3 and bikunin, highlighting the integral involvement of ITIH3 in the structural composition of these plasma protease inhibitors.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q06033 (L35-D651)

Gene ID
Molecular Construction
N-term
ITIH3 (L35-D651)
Accession # Q06033
6*His
C-term
Synonyms
rHuInter-alpha-trypsin inhibitor heavy chain H3/ITIH3/, His; Inter-alpha-trypsin inhibitor heavy chain H3; ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3; Serum-derived hyaluronan-associated protein; SHAP
AA Sequence

LPEGVANGIEVYSTKINSKVTSRFAHNVVTMRAVNRADTAKEVSFDVELPKTAFITNFTLTIDGVTYPGNVKEKEVAKKQYEKAVSQGKTAGLVKASGRKLEKFTVSVNVAAGSKVTFELTYEELLKRHKGKYEMYLKVQPKQLVKHFEIEVDIFEPQGISMLDAEASFITNDLLGSALTKSFSGKKGHVSFKPSLDQQRSCPTCTDSLLNGDFTITYDVNRESPGNVQIVNGYFVHFFAPQGLPVVPKNVAFVIDISGSMAGRKLEQTKEALLRILEDMQEEDYLNFILFSGDVSTWKEHLVQATPENLQEARTFVKSMEDKGMTNINDGLLRGISMLNKAREEHRIPERSTSIVIMLTDGDANVGESRPEKIQENVRNAIGGKFPLYNLGFGNNLNYNFLENMALENHGFARRIYEDSDADLQLQGFYEEVANPLLTGVEMEYPENAILDLTQNTYQHFYDGSEIVVAGRLVDEDMNSFKADVKGHGATNDLTFTEEVDMKEMEKALQERDYIFGNYIERLWAYLTIEQLLEKRKNAHGEEKENLTARALDLSLKYHFVTPLTSMVVTKPEDNEDERAIADKPGEDAEATPVSPAMSYLTSYQPPQNPYYYVDGD

Molecular Weight

Approximately 90.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

ITIH3 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ITIH3 Protein, Human (HEK293, His)
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