Copy Link Print Information

LBP Protein, Human (I465F, HEK293, His)

Customer Review

Based on 1 Customer Validation

LBP proteins play a central role in the innate immune response by directly binding to the lipid A moiety of bacterial lipopolysaccharide (LPS). This interaction enhances CD14 affinity, promotes its binding to LPS, and promotes cytokine release in response to bacterial lipopolysaccharide. LBP Protein, Human (I465F, HEK293, His) is the recombinant human-derived LBP protein, expressed by HEK293 , with C-10*His labeled tag and I465F mutation.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for LBP Protein, Human (I465F, HEK293, His)
  • Experimental Validation Results for LBP Protein, Human (I465F, HEK293, His)
  • LBP Protein, Human (I465F, HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

LBP proteins play a central role in the innate immune response by directly binding to the lipid A moiety of bacterial lipopolysaccharide (LPS). This interaction enhances CD14 affinity, promotes its binding to LPS, and promotes cytokine release in response to bacterial lipopolysaccharide. LBP Protein, Human (I465F, HEK293, His) is the recombinant human-derived LBP protein, expressed by HEK293 , with C-10*His labeled tag and I465F mutation.

Background

LBP takes center stage in the innate immune response by directly engaging with the lipid A moiety of bacterial lipopolysaccharides (LPS), a pivotal glycolipid abundantly present in the outer membrane of Gram-negative bacteria. This interaction serves as a critical affinity enhancer for CD14, promoting its association with LPS and facilitating the subsequent release of cytokines in response to bacterial lipopolysaccharide. Notably, LBP, when bound to LPS, establishes interactions through its C-terminus with both soluble and membrane-bound CD14, underscoring its instrumental role in orchestrating key events in the innate immune system's defense against bacterial pathogens.

Verified Bioactivity

Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is ≤4.892 ng/ml, corresponding to a specific activity is ≥2.04×105 U/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 4.892 ng/mL, corresponding to a specific activity is 2.04×105 U/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    P18428 (A26-V481, I465F)

  • Gene ID
    3929  [NCBI]
  • Molecular Construction
    • N-term
    • LBP (A26-V481, I465F)
      Accession # P18428
    • 10*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    LBP; BPIFD2; Lipopolysaccharide Binding Protein; BPI Fold Containing Family D, Member 2; Lipopolysaccharide-Binding Protein; LPS-Binding Protein

  • AA Sequence

    ANPGLVARITDKGLQYAAQEGLLALQSELLRITLPDFTGDLRIPHVGRGRYEFHSLNIHSCELLHSALRPVPGQGLSLSISDSSIRVQGRWKVRKSFFKLQGSFDVSVKGISISVNLLLGSESSGRPTVTASSCSSDIADVEVDMSGDLGWLLNLFHNQIESKFQKVLESRICEMIQKSVSSDLQPYLQTLPVTTEIDSFADIDYSLVEAPRATAQMLEVMFKGEIFHRNHRSPVTLLAAVMSLPEEHNKMVYFAISDYVFNTASLVYHEEGYLNFSITDDMIPPDSNIRLTTKSFRPFVPRLARLYPNMNLELQGSVPSAPLLNFSPGNLSVDPYMEIDAFVLLPSSSKEPVFRLSVATNVSATLTFNTSKITGFLKPGKVKVELKESKVGLFNAELLEALLNYYILNTFYPKFNDKLAEGFPLPLLKRVQLYDLGLQFHKDFLFLGANVQYMRV

  • Molecular Weight

    Approximately 62 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 4.892 ng/mL, corresponding to a specific activity is 2.04×105 U/mg.

  • LBP Protein, Human (I465F, HEK293, His) Product Image
    1/16
  • Experimental Validation Results for LBP Protein, Human (I465F, HEK293, His)
  • Experimental Validation Results for LBP Protein, Human (I465F, HEK293, His)
  • LBP Protein, Human (I465F, HEK293, His) Product Image