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  4. LCAT Protein, Mouse (HEK293, His)

LCAT Protein, Mouse (HEK293, His)

Cat. No.: HY-P77979
Handling Instructions

LCAT protein, synthesized in the liver and brain, converts cholesterol and phosphatidylcholines on lipoproteins to cholesteryl esters and lysophosphatidylcholines. It transports cholesterol esters to the liver and influences cerebral spinal fluid levels. LCAT also remodels high-density lipoprotein particles, hydrolyzes platelet-activating factor, and esterifies (24S)-hydroxycholesterol to produce 24(S)OH-C monoesters. LCAT Protein, Mouse (HEK293, His) is the recombinant mouse-derived LCAT protein, expressed by HEK293 , with C-His labeled tag. The total length of LCAT Protein, Mouse (HEK293, His) is 414 a.a., with molecular weight of 67-75 kDa.

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Description

LCAT protein, synthesized in the liver and brain, converts cholesterol and phosphatidylcholines on lipoproteins to cholesteryl esters and lysophosphatidylcholines. It transports cholesterol esters to the liver and influences cerebral spinal fluid levels. LCAT also remodels high-density lipoprotein particles, hydrolyzes platelet-activating factor, and esterifies (24S)-hydroxycholesterol to produce 24(S)OH-C monoesters. LCAT Protein, Mouse (HEK293, His) is the recombinant mouse-derived LCAT protein, expressed by HEK293 , with C-His labeled tag. The total length of LCAT Protein, Mouse (HEK293, His) is 414 a.a., with molecular weight of 67-75 kDa.

Background

The LCAT protein is a central enzyme involved in the extracellular metabolism of plasma lipoproteins. It is primarily synthesized in the liver and secreted into the bloodstream, where it converts cholesterol and phosphatidylcholines to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). This process facilitates the transport of cholesterol esters back to the liver. In addition to its hepatic production, LCAT is also produced in the brain by primary astrocytes. In the brain, it esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia, influencing cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, LCAT plays a crucial role in the maturation of glial-derived, nascent lipoproteins. Furthermore, LCAT is responsible for remodeling high-density lipoprotein particles into their spherical forms. It exhibits a preference for specific phosphatidylcholines in the plasma and can also catalyze the hydrolysis of platelet-activating factor (PAF) and transfer of the acetate group from PAF to another molecule. Additionally, LCAT catalyzes the esterification of (24S)-hydroxycholesterol to produce 24(S)OH-C monoesters.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

P16301 (F25-E438)

Gene ID

16816  [NCBI]

Molecular Construction
N-term
LCAT (F25-E438)
Accession # P16301
His
C-term
Synonyms
PAF acetylhydrolase; LCAT
Molecular Weight

67-75 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

LCAT Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LCAT Protein, Mouse (HEK293, His)
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HY-P77979
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