1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. CSF & Receptors
  4. M-CSF & Receptors
  5. M-CSF Protein, Human (HEK293, His)

M-CSF Protein, Human (HEK293, His)

Cat. No.: HY-P73827
Handling Instructions
Top Publications Citing Use of Products

M-CSF Protein, Human (HEK293, His) Related Classifications

  • Technical Parameters

  • Properties

  • Documentation

Species

Human

Source

HEK293

Tag

C-His

Accession

P09603 (M1-N190)

Gene ID

1435

Synonyms
Macrophage Colony-Stimulating Factor 1; CSF-1; M-CSF; Lanimostim
AA Sequence

Molecular Weight

27-32 kDa

Purity

Greater than 95% as determined by SDS-PAGE.

Biological Activity
Immobilized human CSF1R at 10 μg/mL (100 μL/well) can bind human CSF-1 and the EC50 is 8 ng/mL. Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells and the ED50 is typically 2-8 ng/mL.
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
M-CSF Protein, Human (HEK293, His)
Cat. No.:
HY-P73827
Quantity:
MCE Japan Authorized Agent: