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IL-34 Protein, Human (CHO, His)

1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

IL-34 protein is an important cytokine that promotes the proliferation, survival, and differentiation of monocytes and macrophages. It plays a key role in innate immune responses and inflammatory processes by promoting the release of pro-inflammatory chemokines. IL-34 Protein, Human (CHO, His) is the recombinant human-derived IL-34 protein, expressed by CHO , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: CHO
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-34 Protein, Human (CHO, His)
  • Experimental Validation Results for IL-34 Protein, Human (CHO, His)
  • IL-34 Protein, Human (CHO, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

IL-34 protein is an important cytokine that promotes the proliferation, survival, and differentiation of monocytes and macrophages. It plays a key role in innate immune responses and inflammatory processes by promoting the release of pro-inflammatory chemokines. IL-34 Protein, Human (CHO, His) is the recombinant human-derived IL-34 protein, expressed by CHO , with C-His labeled tag.

Background

Interleukin-34 (IL-34) is a cytokine with pivotal functions in innate immunity and inflammatory processes, as it promotes the proliferation, survival, and differentiation of monocytes and macrophages. Additionally, IL-34 plays a crucial role in the regulation of osteoclast proliferation and differentiation, influencing bone resorption. The cytokine's impact is mediated through its interaction with CSF1R, leading to the activation of downstream effectors and subsequent phosphorylation of MAPK1/ERK2 and MAPK3/ERK1. Structurally, IL-34 exists as a homodimer, and its intricate involvement in immune regulation and bone homeostasis highlights its significance in orchestrating cellular responses and maintaining tissue integrity.

Verified Bioactivity

Measured in a cell proliferation assay using human peripheral blood mononuclear cell and the ED50 is 2-8 ng/mL.

Technical Parameters

  • Species Human
  • Source CHO
  • Tag C-His
  • Accession

    Q6ZMJ4-1/AAH29804.1 (N21-P242)

  • Gene ID
    146433  [NCBI]
  • Molecular Construction
    • N-term
    • IL-34 (N21-P242)
      Accession # Q6ZMJ4-1/AAH29804.1
    • His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    IL34; Interleukin-34; Prev. C16orf77; Chromosome 16 Open Reading Frame 77; IL-34; Interleukin 34; MGC34647

  • AA Sequence

    NEPLEMWPLTQNEECTVTGFLRDKLQYRSRLQYMKHYFPINYKISVPYEGVFRIANVTRLQRAQVSERELRYLWVLVSLSATESVQDVLLEGHPSWKYLQEVETLLLNVQQGLTDVEVSPKVESVLSLLNAPGPNLKLVRPKALLDNCFRVMELLYCSCCKQSSVLNWQDCEVPSPQSCSPEPSLQYAATQLYPPPPWSPSSPPHSTGSVRPVRAQGEGLLP

  • Predicted Molecular Mass

    26.7 kDa

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    IL-34 Protein, Human (CHO, His) purchased from MedChemExpress. Usage Cited in: Mol Immunol. 2025 Jan 25:178:64-75. [Abstract]

    Customer Validation
     IL-34 Protein, Human (CHO, His) (100 ng/mL) increased the protein expression of ZO-1 and Occludin in NCM460 cells.

  • 1/16

    IL-34 Protein, Human (CHO, His) purchased from MedChemExpress. Usage Cited in: Mol Immunol. 2025 Jan 25:178:64-75. [Abstract]

    Customer Validation
     IL-34 Protein, Human (CHO, His) (10, 50, 100, 200 ng/mL) increased the protein expression of ZO-1 and Occludin in a dose-dependent manner in NCM460 cells.

  • IL-34 Protein, Human (CHO, His) Product Image
    1/16
  • Experimental Validation Results for IL-34 Protein, Human (CHO, His)
  • Experimental Validation Results for IL-34 Protein, Human (CHO, His)
  • IL-34 Protein, Human (CHO, His) Product Image

  • IL-34 Protein, Human (CHO, His) purchased from MedChemExpress. Usage Cited in: Mol Immunol. 2025 Jan 25:178:64-75. [Abstract]

    IL-34 Protein, Human (CHO, His) (100 ng/mL) increased the protein expression of ZO-1 and Occludin in NCM460 cells.

  • IL-34 Protein, Human (CHO, His) purchased from MedChemExpress. Usage Cited in: Mol Immunol. 2025 Jan 25:178:64-75. [Abstract]

    IL-34 Protein, Human (CHO, His) (10, 50, 100, 200 ng/mL) increased the protein expression of ZO-1 and Occludin in a dose-dependent manner in NCM460 cells.