MAP3K8/TPL-2 Protein, Human (sf9, GST)
MAP3K8/TPL-2 is critical for LPS-induced, TLR4-mediated activation of MAPK/ERK, leading to TNF production by macrophages. It regulates T cell differentiation, IFNG expression, and host resistance to bacterial infections by negatively regulating type I interferon production. MAP3K8/TPL-2 Protein, Human (sf9, GST) is the recombinant human-derived MAP3K8/TPL-2 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
MAP3K8/TPL-2 is critical for LPS-induced, TLR4-mediated activation of MAPK/ERK, leading to TNF production by macrophages. It regulates T cell differentiation, IFNG expression, and host resistance to bacterial infections by negatively regulating type I interferon production. MAP3K8/TPL-2 Protein, Human (sf9, GST) is the recombinant human-derived MAP3K8/TPL-2 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
Background
MAP3K8/TPL-2 is crucial for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, playing a pivotal role in the production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. It also participates in the regulation of T-helper cell differentiation and IFNG expression in T-cells, contributing to the modulation of host resistance against bacterial infections by negatively regulating type I interferon (IFN) production. Furthermore, MAP3K8/TPL-2 is involved in transducing signals from CD40 and TNFRSF1A, activating ERK in B-cells and macrophages, and potentially influencing immunoglobulin production. In adipocytes, it activates the MAPK/ERK pathway in response to IL1B and TNF, contributing to the induction of lipolysis in an IKBKB-dependent manner. Additionally, MAP3K8/TPL-2 may play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in specific cell types. Its involvement in the cell cycle is evident, with isoform 1 exhibiting some transforming activity, albeit weaker than that of the activated oncogenic variant.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
P41279 (M30-R397)
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Molecular Construction
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N-term
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GST
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MAP3K8 (M30-R397)
Accession # P41279 -
C-term
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Protein Length
Partial
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Synonyms
MAP3K8; Proto-Oncogene Serine/Threoine Protein Kinase; Prev. ESTF; Cot (Cancer Osaka Thyroid) Oncogene; Prev. COT; Serine/Threonine-Protein Kinase Cot; Tumor Progression Locus 2; Cancer Osaka Thyroid Oncogene; Tpl-2; Ewing Sarcoma Transformant; C-COT; AUR
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AA Sequence
MENLYASEEPAVYEPSLMTMCQDSNQNDERSKSLLLSGQEVPWLSSVRYGTVEDLLAFANHISNTAKHFYGQRPQESGILLNMVITPQNGRYQIDSDVLLIPWKLTYRNIGSDFIPRGAFGKVYLAQDIKTKKRMACKLIPVDQFKPSDVEIQACFRHENIAELYGAVLWGETVHLFMEAGEGGSVLEKLESCGPMREFEIIWVTKHVLKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGLSVQMTEDVYFPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYLYIIHKQAPPLEDIADDCSPGMRELIEASLERNPNHRPRAADLLKHEALNPPREDQPR
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Predicted Molecular Mass
68 kDa
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Molecular Weight
Approximately 68 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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