PKMYT1 Protein, Human (Active, sf9, His)
PKMYT1 Protein, Human (sf9, His) is the recombinant human-derived MYT1, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
PKMYT1 Protein, Human (sf9, His) is the recombinant human-derived MYT1, expressed by Sf9 insect cells , with N-His labeled tag.
Background
MYT1 acts as a negative regulator of mitotic entry (G2 to M transition) by phosphorylating CDK1 kinase specifically when complexed with cyclins. It predominantly mediates phosphorylation of CDK1 at 'Thr-14'. Additionally, MYT1 is involved in Golgi fragmentation. It may also contribute to CDK1 phosphorylation at 'Tyr-15' to a lesser extent, though its tyrosine kinase activity remains unclear and potentially indirect (by similar).
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-8*His
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Accession
Q99640-1 (M1-T499)
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Gene ID9088
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Molecular Construction
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N-term
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GST
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MYT1 (M1-T499)
Accession # Q99640-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
PKMYT1; Myt1 Kinase; Protein Kinase, Membrane Associated Tyrosine/Threonine 1; PPP1R126; Membrane-Associated Tyrosine- And Threonine-Specific Cdc2-Inhibitory Kinase; Protein Phosphatase 1, Regulatory Subunit 126; MYT1; Non-Specific Serine/Threonine Protein Kinase
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AA Sequence
MLERPPALAMPMPTEGTPPPLSGTPIPVPAYFRHAEPGFSLKRPRGLSRSLPPPPPAKGSIPISRLFPPRTPGWHQLQPRRVSFRGEASETLQSPGYDPSRPESFFQQSFQRLSRLGHGSYGEVFKVRSKEDGRLYAVKRSMSPFRGPKDRARKLAEVGSHEKVGQHPCCVRLEQAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQVWGYLRDTLLALAHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGTAGAGEVQEGDPRYMAPELLQGSYGTAADVFSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLVMMLEPDPKLRATAEALLALPVLRQPRAWGVLWCMAAEALSRGWALWQALLALLCWLWHGLAHPASWLQPLGPPATPPGSPPCSLLLDSSLSSNWDDDSLGPSLSPEAVLARTVGSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLLSLFEDTLDPT
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Predicted Molecular Mass
58.7 kDa
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Molecular Weight
Approximately 65 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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