NAMPT Protein, Mouse

NAMPT protein has dual functions of cytokine and adipokine. Due to limited ATP activation, its secreted form lacks enzymatic activity. It catalyzes the condensation of nicotinamide and 5-phosphoribosyl-1-pyrophosphate to produce nicotinamide mononucleotide, which is critical for NAD biosynthesis. NAMPT Protein, Mouse is the recombinant mouse-derived NAMPT protein, expressed by E. coli , with tag free. The total length of NAMPT Protein, Mouse is 491 a.a., with molecular weight of 56 kDa.

The NAMPT protein exhibits dual functionality, acting both as a cytokine with immunomodulating properties and as an adipokine with anti-diabetic properties. Interestingly, its secreted form lacks enzymatic activity, attributed in part to the limited activation by ATP in the extracellular space and plasma due to its low levels. Functionally, NAMPT catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate, yielding nicotinamide mononucleotide—an intermediate crucial in the biosynthesis of NAD. As the rate-limiting component in the mammalian NAD biosynthesis pathway, NAMPT plays a pivotal role in modulating circadian clock function. Its NAMPT-dependent oscillatory production of NAD governs the oscillation of clock target gene expression by releasing the core clock component, the CLOCK-BMAL1 heterodimer, from NAD-dependent SIRT1-mediated suppression.

Mouse

E. coli

Tag Free

Q99KQ4 (M1-H491)

59027

56 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.