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Neurotrophin-3 Protein, Human

2 Cited Publications
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Based on 2 publication(s) in Google Scholar

Neurotrophin-3 Protein, Human is a recombinant Neurotrophin-3 protein expressed in E. coli system. Neurotrophin-3 is widely expressed in the nervous system. Neurotrophin-3 reduces cellular damage, improves neuronal regeneration in different models of lesions.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Neurotrophin-3 Protein, Human Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Neurotrophin-3 Protein, Human is a recombinant Neurotrophin-3 protein expressed in E. coli system. Neurotrophin-3 is widely expressed in the nervous system. Neurotrophin-3 reduces cellular damage, improves neuronal regeneration in different models of lesions[1].

Background

Neurotrophin-3 (NT-3), a trophic factor from the neurotrophin family, is widely expressed in the nervous system.
The physiological actions of NT-3 are mediated by the activation of two membrane receptors: the low-affinity receptor p75 and the high-affinity receptor Trk. NT-3 activates the TrkC receptor and binds with less affinity to TrkB and TrkA.
NT-3 reduces cellular damage, improves neuronal regeneration in different models of lesions or neurodegeneration, and participates in synaptic reorganization, synapse formation, and neuronal plasticity[1].

In Vitro

Neurotrophin-3 (human; 2 ng/mL; 20 h), but not brain-derived neurotrophic factor, promotes neuronal differentiation of retinal progenitor E4 cells[3].

Verified Bioactivity

1.The ED50 as determined by its ability to bind Human NTRK2 in functional ELISA is less than 10 μg/mL.
2.Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED50 for this effect is 33.69-35.88 ng/mL, corresponding to a specific activity is 2.787-2.968×104 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED50 for this effect is 35.88 ng/mL, corresponding to a specific activity is 2.787×104 units/mg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P20783-1 (Y139-T257)

  • Gene ID
    4908  [NCBI]
  • Molecular Construction
    • N-term
    • Neurotrophin-3 (Y139-T257)
      Accession # P20783-1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    NTF3; Neurotrophin-3; Neurotrophin 3; NGF-2; NGF2; HDNF; Nerve Growth Factor 2; NT-3; Neurotrophic Factor; NT3

  • AA Sequence

    YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

  • Predicted Molecular Mass

    13.6 kDa

  • Molecular Weight

    Approximately 14 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.2.
2.Lyophilized from a 0.22 μm filtered solution of 0.1% TFA, 20% Acetonitrile, pH 2.5.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Koichi Uegaki, et al. BDNF Binds Its Pro-Peptide with High Affinity and the Common Val66 Met Polymorphism Attenuates the Interaction. Int J Mol Sci. 2017 May 12;18(5):1042. [Content Brief]

[2]. O Blondel, et al. A glia-derived signal regulating neuronal differentiation. J Neurosci. 2000 Nov 1;20(21):8012-20. [Content Brief]

[3]. de la Rosa EJ, et al. Role of neurotrophins in the control of neural development: neurotrophin-3 promotes both neuron differentiation and survival of cultured chick retinal cells. Neuroscience. 1994 Jan;58(2):347-52. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED50 for this effect is 35.88 ng/mL, corresponding to a specific activity is 2.787×104 units/mg.

  • 1/16

    Neurotrophin-3 Protein, Human purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Jul 5;10(27):eado9120. [Abstract]

    Customer Validation
     In vitro release rates of Neurotrophin-3 Protein and Cur from the HADA/HRR hydrogel.

  • 1/16

    Neurotrophin-3 Protein, Human purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Jul 5;10(27):eado9120. [Abstract]

    Customer Validation
     Representative images of longitudinal spinal cord sections obtained at 2 wpi in the CTRL and HADA/HRR + Neurotrophin-3/Cur hydrogel group. Dotted lines demarcated astrocyte border around the lesion. Boxed border regions were magnified with NF200 and different ECM [laminin (Ln), collagen I (Col 1), and fibronectin (Fn)] immunoreactivity intermingled in the region.

  • 1/16

    Neurotrophin-3 Protein, Human purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Jul 5;10(27):eado9120. [Abstract]

    Customer Validation
     Immunostainings for glial fibrillary acidic protein (GFAP) and CSPG of the HADA/HRR + Neurotrophin-3/Cur hydrogel group at 2 wpi. The boxed border regions were magnified and immunostained with Ln and NF200 (i1), CSPG and NF200 (i2) or CSPG, Ln, and NF200 (i3).

  • 1/16

    Neurotrophin-3 Protein, Human purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Jul 5;10(27):eado9120. [Abstract]

    Customer Validation
     Images of immunostaining for Ln and NF200 in the HADA/HRR + Neurotrophin-3/Cur hydrogel group at 8 wpi. The boxed border regions were magnified in different groups.

  • 1/16

    Neurotrophin-3 Protein, Human purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Jul 5;10(27):eado9120. [Abstract]

    Customer Validation
     Representative images of longitudinal spinal cord sections at 2 wpi in the CTRL and HADA/HRR + Neurotrophin-3/Cur hydrogel group, respectively. The boxed border regions were magnified with Ln and PDGFRβ immunoreactivity in the region.

  • Neurotrophin-3 Protein, Human Product Image
    1/16
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Experimental Validation Results for Neurotrophin-3 Protein, Human
  • Neurotrophin-3 Protein, Human Product Image

  • Neurotrophin-3 Protein, Human purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Jul 5;10(27):eado9120. [Abstract]

    In vitro release rates of Neurotrophin-3 Protein and Cur from the HADA/HRR hydrogel.

  • Neurotrophin-3 Protein, Human purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Jul 5;10(27):eado9120. [Abstract]

    Representative images of longitudinal spinal cord sections obtained at 2 wpi in the CTRL and HADA/HRR + Neurotrophin-3/Cur hydrogel group. Dotted lines demarcated astrocyte border around the lesion. Boxed border regions were magnified with NF200 and different ECM [laminin (Ln), collagen I (Col 1), and fibronectin (Fn)] immunoreactivity intermingled in the region.

  • Neurotrophin-3 Protein, Human purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Jul 5;10(27):eado9120. [Abstract]

    Immunostainings for glial fibrillary acidic protein (GFAP) and CSPG of the HADA/HRR + Neurotrophin-3/Cur hydrogel group at 2 wpi. The boxed border regions were magnified and immunostained with Ln and NF200 (i1), CSPG and NF200 (i2) or CSPG, Ln, and NF200 (i3).

  • Neurotrophin-3 Protein, Human purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Jul 5;10(27):eado9120. [Abstract]

    Images of immunostaining for Ln and NF200 in the HADA/HRR + Neurotrophin-3/Cur hydrogel group at 8 wpi. The boxed border regions were magnified in different groups.

  • Neurotrophin-3 Protein, Human purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Jul 5;10(27):eado9120. [Abstract]

    Representative images of longitudinal spinal cord sections at 2 wpi in the CTRL and HADA/HRR + Neurotrophin-3/Cur hydrogel group, respectively. The boxed border regions were magnified with Ln and PDGFRβ immunoreactivity in the region.