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NAMPT Protein, Human (His)

5 Cited Publications
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Based on 5 publication(s) in Google Scholar

NAMPT protein catalyzes the synthesis of nicotinamide mononucleotide, a key intermediate in NAD biosynthesis. NAMPT Protein, Human (His) is the recombinant human-derived NAMPT protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • NAMPT Protein, Human (His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

NAMPT protein catalyzes the synthesis of nicotinamide mononucleotide, a key intermediate in NAD biosynthesis. NAMPT Protein, Human (His) is the recombinant human-derived NAMPT protein, expressed by E. coli , with N-6*His labeled tag.

Background

NAMPT serves as the catalyst for the condensation reaction between nicotinamide and 5-phosphoribosyl-1-pyrophosphate, giving rise to nicotinamide mononucleotide—a pivotal intermediate in the biosynthesis of NAD. As the rate-limiting factor in the mammalian NAD biosynthetic pathway, NAMPT holds a central role in cellular processes. Beyond its enzymatic function, the secreted form of NAMPT exhibits dual properties, acting as both a cytokine with immunomodulating effects and an adipokine with anti-diabetic attributes. Notably, its extracellular function lacks enzymatic activity, partially due to the absence of activation by ATP, which is present at low levels in the extracellular space and plasma. Moreover, NAMPT plays a crucial role in the modulation of circadian clock function, orchestrating the oscillatory production of NAD to regulate clock target gene expression by facilitating the release of the CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.

Verified Bioactivity

1.Measured by its ability to convert PRPP to NMN. The specific activity is 15.13987 pmol/min/µg, as measured under the described conditions.
2.Measured by its ability to induce VEGF secretion by PC-3 human prostate cancer cells. The ED50 for this effect is 0.5-2.0 μg/mL.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    P43490 (M1-H491)

  • Gene ID
    10135  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • NAMPT (M1-H491)
      Accession # P43490
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    NAMPT; Pre-B-Cell Colony-Enhancing Factor 1; Prev. PBEF1; Pre-B-Cell Colony Enhancing Factor 1; Visfatin; 1110035O14Rik; PBEF; Nampt; NAmPRTase; VF; Nicotinamide Phosphoribosyltransferase; Pre-B Cell-Enhancing Factor

  • AA Sequence

    MNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH

  • Molecular Weight

    Approximately 55 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 10% trehalose, 100 mM Arg-HCl, 0.02% Tween 80, pH 6.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 10% trehalose, 0.02% Tween 80.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 8.0, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce VEGF secretion by PC-3 human prostate cancer cells. The ED50 for this effect is 0.7365 μg/mL, corresponding to a specific activity is 1.36×103 U/mg.

  • 1/16

    NAMPT Protein, Human (His) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar;12(12):e2412282. [Abstract]

    Customer Validation
     Analysis of protein expression levels for collagen‐1 (Col‐1), fibronectin (FN), and GAPDH following stimulation with exogenous NAMPT Protein, Human, demonstrating the fibrogenic response.

  • 1/16

    NAMPT Protein, Human (His) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar;12(12):e2412282. [Abstract]

    Customer Validation
     NAMPT Protein, Human (0.4 mg/kg). Representative images show DCFH-DA and DAPI staining in the pancreas of AIP mice, compared with normal control mice.

  • 1/16

    NAMPT Protein, Human (His) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar;12(12):e2412282. [Abstract]

    Customer Validation
     The protein expression levels of collagen-1 (Col-1), fibronectin (FN), and GAPDH in HPSCs from the control group and the TLR4 knockdown group were evaluated after treatment with exogenous NAMPT Protein, Human (0.4 mg/kg).

  • 1/16

    NAMPT Protein, Human (His) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar;12(12):e2412282. [Abstract]

    Customer Validation
     Representative Masson-stained images showing pancreatic structures in AIP mice treated with or without exogenous NAMPT Protein, Human (0.4 mg/kg), or FK866.

  • 1/16

    NAMPT Protein, Human (His) purchased from MedChemExpress. Usage Cited in: J Cachexia Sarcopenia Muscle. 2023 Apr;14(2):1003-1018. [Abstract]

    Customer Validation
     Western blot analysis of NMRK2 and NAMPT Protein, Human (2.0 μg) in supraspinatus muscles. GAPDH is used as loading control.

  • NAMPT Protein, Human (His) Product Image
    1/16
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • Experimental Validation Results for NAMPT Protein, Human (His)
  • NAMPT Protein, Human (His) Product Image