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NAMPT Protein, Human (His)

Cat. No.: HY-P70353
COA Handling Instructions

NAMPT protein catalyzes the synthesis of nicotinamide mononucleotide, a key intermediate in NAD biosynthesis. NAMPT Protein, Human (His) is the recombinant human-derived NAMPT protein, expressed by E. coli , with N-6*His labeled tag. The total length of NAMPT Protein, Human (His) is 491 a.a., with molecular weight of 55-57 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

NAMPT protein catalyzes the synthesis of nicotinamide mononucleotide, a key intermediate in NAD biosynthesis. NAMPT Protein, Human (His) is the recombinant human-derived NAMPT protein, expressed by E. coli , with N-6*His labeled tag. The total length of NAMPT Protein, Human (His) is 491 a.a., with molecular weight of 55-57 kDa.

Background

NAMPT serves as the catalyst for the condensation reaction between nicotinamide and 5-phosphoribosyl-1-pyrophosphate, giving rise to nicotinamide mononucleotide—a pivotal intermediate in the biosynthesis of NAD. As the rate-limiting factor in the mammalian NAD biosynthetic pathway, NAMPT holds a central role in cellular processes. Beyond its enzymatic function, the secreted form of NAMPT exhibits dual properties, acting as both a cytokine with immunomodulating effects and an adipokine with anti-diabetic attributes. Notably, its extracellular function lacks enzymatic activity, partially due to the absence of activation by ATP, which is present at low levels in the extracellular space and plasma. Moreover, NAMPT plays a crucial role in the modulation of circadian clock function, orchestrating the oscillatory production of NAD to regulate clock target gene expression by facilitating the release of the CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.

Biological Activity

1.Measured by its ability to convert PRPP to NMN. The specific activity is 15.13987 pmol/min/µg, as measured under the described conditions.
2.Measured by its ability to induce VEGF secretion by PC‑3 human prostate cancer cells. The ED 50 for this effect is 0.7504 μg/mL, corresponding to a specific activity is 1.333×103 U/mg.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

P43490 (M1-H491)

Gene ID
Molecular Construction
N-term
6*His
NAMPT (M1-H491)
Accession # P43490
C-term
Synonyms
rHuNicotinamide phosphoribosyltransferase/NAMPT, His; Pre-B cell-enhancing factor; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Visfatin; NAMPT; PBEF; PBEF1
AA Sequence

MNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH

Molecular Weight

Approximately 55-57 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 8.0 or PBS, pH 7.4, 10% trehalose and 0.02% Tween80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

NAMPT Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
NAMPT Protein, Human (His)
Cat. No.:
HY-P70353
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