Copy Link Print Information

OGN/Osteoglycin Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

OGN/osteoglycinin cooperates with TGF-β-1 or TGF-β-2 to play a crucial role in bone formation. Its synergistic interaction with these growth factors emphasizes its importance in regulating bone homeostasis and development. OGN/Osteoglycin Protein, Human (HEK293, His) is the recombinant human-derived OGN/Osteoglycin protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • OGN/Osteoglycin Protein, Human (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

OGN/osteoglycinin cooperates with TGF-β-1 or TGF-β-2 to play a crucial role in bone formation. Its synergistic interaction with these growth factors emphasizes its importance in regulating bone homeostasis and development. OGN/Osteoglycin Protein, Human (HEK293, His) is the recombinant human-derived OGN/Osteoglycin protein, expressed by HEK293 , with C-His labeled tag.

Background

OGN/Osteoglycin protein plays a crucial role in bone formation, collaborating with TGF-beta-1 or TGF-beta-2 to induce this process. Its functional synergy with these transforming growth factors underscores its significance in the regulation of bone homeostasis and development. By participating in the intricate signaling pathways involving TGF-beta isoforms, OGN contributes to the orchestration of molecular events that culminate in the formation and maintenance of bone tissue. The collaborative action of OGN with TGF-beta highlights its role as a modulator of bone formation processes, offering potential insights into the regulatory mechanisms underlying skeletal health and physiology.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    P20774 (P21-F298)

  • Gene ID
    4969  [NCBI]
  • Molecular Construction
    • N-term
    • OGN (P21-F298)
      Accession # P20774
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    OGN; Osteoinductive Factor; Osteoglycin; Mimecan Proteoglycan; Mimecan; Corneal Keratan Sulfate Proteoglycan; SLRR3A; Osteoglycin (Osteoinductive Factor); OIF; OG

  • AA Sequence

    PPTQQDSRIIYDYGTDNFEESIFSQDYEDKYLDGKNIKEKETVIIPNEKSLQLQKDEAITPLPPKKENDEMPTCLLCVCLSGSVYCEEVDIDAVPPLPKESAYLYARFNKIKKLTAKDFADIPNLRRLDFTGNLIEDIEDGTFSKLSLLEELSLAENQLLKLPVLPPKLTLFNAKYNKIKSRGIKANAFKKLNNLTFLYLDHNALESVPLNLPESLRVIHLQFNNIASITDDTFCKANDTSYIRDRIEEIRLEGNPIVLGKHPNSFICLKRLPIGSYF

  • Predicted Molecular Mass

    32.8 kDa

  • Molecular Weight

    Approximately 45-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • OGN/Osteoglycin Protein, Human (HEK293, His) Product Image
    1/16
  • OGN/Osteoglycin Protein, Human (HEK293, His) Product Image