OXSR1 Protein, Human (sf9, His-GST)
The OXSR1 protein is a key serine/threonine protein kinase in the WNK-SPAK/OSR1 cascade, regulating ion transport, hyperosmotic stress response, and blood pressure. Downstream of WNK kinase, OXSR1 phosphorylates ion cotransporters such as SLC12A1/NKCC2, SLC12A2/NKCC1, and SLC12A3/NCC, regulating their activity. OXSR1, Human (sf9, His-GST) is the recombinant human-derived OXSR1 protein, expressed by sf9 insect cells, with N-Avi labeled tag.
- Species: Human
- Source: sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
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Biological Activity
Description
The OXSR1 protein is a key serine/threonine protein kinase in the WNK-SPAK/OSR1 cascade, regulating ion transport, hyperosmotic stress response, and blood pressure. Downstream of WNK kinase, OXSR1 phosphorylates ion cotransporters such as SLC12A1/NKCC2, SLC12A2/NKCC1, and SLC12A3/NCC, regulating their activity. OXSR1, Human (sf9, His-GST) is the recombinant human-derived OXSR1 protein, expressed by sf9 insect cells, with N-Avi labeled tag.
Background
OXSR1 Protein functions as a key effector serine/threonine-protein kinase in the WNK-SPAK/OSR1 cascade, participating in diverse cellular processes such as ion transport, response to hypertonic stress, and blood pressure regulation. It recognizes and binds proteins with a RFXV motif, acting downstream of WNK kinases to phosphorylate ion cotransporters including SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2, and SLC12A6/KCC3, thereby modulating their activity. OXSR1 mediates regulatory volume increase in response to hyperosmotic stress and regulates NaCl reabsorption in the distal nephron by phosphorylating the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC. Additionally, it serves as a regulator of angiogenesis in endothelial cells and activates inward rectifier potassium channels KCNJ2/Kir2.1 and KCNJ4/Kir2.3 downstream of WNK1, influencing their membrane localization. OXSR1 also plays a role in phosphorylating various substrates, including RELL1, RELL2, RELT, PAK1, and PLSCR1 in the presence of RELT.
Technical Parameters
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Species Human
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Source sf9 insect cells
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Tag N-His;N-GST
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Accession
O95747 (S2-S527)
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Gene ID9943
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Molecular Construction
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N-term
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His-GST
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(S2-S527)
Accession # O95747 -
C-term
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Protein Length
Full Length
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Synonyms
OXSR1; KIAA1101; Prev. OSR1; Oxidative Stress Responsive 1; Oxidative Stress-Responsive 1 Protein; Alternative Protein OXSR1; Serine/Threonine-Protein Kinase OSR1; Oxidative Stress Responsive Kinase 1; Oxidative-Stress Responsive 1
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AA Sequence
SEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDESTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQEKTLQRAPTISERAKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEGKAAISQLRSPRVKESISNSELFPTTDPVGTLLQVPEQISAHLPQPAGQIATQPTQVSLPPTAEPAKTAQALSSGSGSQETKIPISLVLRLRNSKKELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipped with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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