PARP2 Protein, Human (sf9, His)
Based on 1 Customer Validation
PARP2 Protein, Human (sf9, His) is the recombinant human-derived PARP2, expressed by Sf9 insect cells , with N-6*His labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
PARP2 Protein, Human (sf9, His) is the recombinant human-derived PARP2, expressed by Sf9 insect cells , with N-6*His labeled tag. ,
PARP2 is a poly-ADP-ribosyltransferase that mediates protein poly-ADP-ribosylation and plays a key role in DNA repair. It catalyzes glutamate, aspartate, or serine ADP-ribosylation, transferring the ADP-D-ribosyl group from NAD(+) to target protein carboxyl groups and forming polymers with an average length of 20-30 units. Serine ADP-ribosylation is the primary form in DNA damage response. Without HPF1, PARP2 mediates glutamate and aspartate ADP-ribosylation, while HPF1 binding confers serine specificity. PARP2 recognizes DNA breaks in chromatin, recruits HPF1, and promotes serine ADP-ribosylation of histones, decompacting chromatin and recruiting repair factors. HPF1 restricts PARP2's polymerase activity to limit poly-ADP-ribose chain length. PARP2 also specifically mediates branched poly-ADP-ribosylation, recognized by factors like APLF. Additionally, PARP2 can ADP-ribosylate DNA, preferentially acting on 5'-terminal phosphates at DNA strand breaks in nicked duplexes.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-6*His
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Accession
Q9UGN5 (M1-W583)
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Gene ID10038
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Protein Length
Full Length
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Synonyms
PARP2; Poly[ADP-Ribose] Synthase 2; Prev. ADPRTL2; ADPRT-2; ARTD2; HPARP-2; ADP-Ribosyltransferase (NAD+; Poly(ADP-Ribose) Polymerase)-Like 2; ADPRT2; ADP-Ribosyltransferase Diphtheria Toxin-Like 2; PARP-2; Poly (ADP-Ribose) Polymerase Family, Member 2; P
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AA Sequence
MAARRRRSTGGGRARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESKKMPVAGGKANKDRTEDKQDGMPGRSWASKRVSESVKALLLKGKAPVDPECTAKVGKAHVYCEGNDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWMRWGRVGKMGQHSLVACSGNLNKAKEIFQKKFLDKTKNNWEDREKFEKVPGKYDMLQMDYATNTQDEEETKKEESLKSPLKPESQLDLRVQELIKLICNVQAMEEMMMEMKYNTKKAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRALMEACNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTELQSPEHPLDQHYRNLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVEKDGEKEAFREDLHNRMLLWHGSRMSNWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEANPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYIVYNPNQVRMRYLLKVQFNFLQLW
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Predicted Molecular Mass
71.6 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4 .
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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