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PARP1 Protein, Human (sf9, His)

8 Cited Publications
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Based on 8 publication(s) in Google Scholar

The PARP1 protein is a polyADP-ribosyltransferase that crucially mediates polyADP-ribosylation (PARsylation) in DNA repair, including base excision and double-strand breaks. By interacting with factors such as HPF1 and NMNAT1, the specificity of PARP1 extends to various amino acids. PARP1 Protein, Human (sf9, His) is the recombinant human-derived PARP1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

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  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for PARP1 Protein, Human (sf9, His)
  • Experimental Validation Results for PARP1 Protein, Human (sf9, His)
  • PARP1 Protein, Human (sf9, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

The PARP1 protein is a polyADP-ribosyltransferase that crucially mediates polyADP-ribosylation (PARsylation) in DNA repair, including base excision and double-strand breaks. By interacting with factors such as HPF1 and NMNAT1, the specificity of PARP1 extends to various amino acids. PARP1 Protein, Human (sf9, His) is the recombinant human-derived PARP1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

PARP1, a poly-ADP-ribosyltransferase, is a crucial mediator of poly-ADP-ribosylation (PARsylation) of proteins, playing a pivotal role in DNA repair, including base excision repair and double-strand breaks repair. Specificity for various amino acids is conferred by interacting factors like HPF1 and NMNAT1. In the context of DNA damage, PARP1 recognizes and binds DNA breaks, recruiting HPF1 and initiating serine ADP-ribosylation of target proteins such as histones. This PARsylation facilitates chromatin decompaction, recruitment of repair factors, and ultimately DNA strand break repair. Additionally, PARP1 is implicated in transcription regulation, acting both as a positive and negative regulator of transcription elongation. It also contributes to processes like replication fork progression, parthanatos, negative regulation of the cGAS-STING pathway, and inhibition of adipogenesis. Furthermore, PARP1 generates nuclear ATP in collaboration with NMNAT1, PARG, and NUDT5, promoting extensive chromatin remodeling. In apoptotic contexts, cleaved and translocated PARP1 serves as a poly-ADP-ribose carrier, inducing AIFM1-mediated apoptosis. The multifaceted functions of PARP1 highlight its integral role in diverse cellular processes.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-His
  • Accession

    P09874 (M1-W1014)

  • Gene ID
    142  [NCBI]
  • Molecular Construction
    • N-term
    • PARP1 (M1-W1014)
      Accession # P09874
    • His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    PARP1; NAD(+) ADP-Ribosyltransferase 1; Prev. ADPRT; Poly[ADP-Ribose] Synthase 1; Prev. PPOL; ADPRT 1; ARTD1; PARP-1; Poly-PARP; ADP-Ribosyltransferase NAD(+); PARS; NAD(+) ADP-Ribosyltransferase; PARP; Poly(ADP-Ribosyl)Transferase; ADP-Ribosyltransferase

  • AA Sequence

    MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW

  • Predicted Molecular Mass

    114.5 kDa

  • Molecular Weight

    Approximately 100-110 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5, 2 mM EDTA, 0.5 mM TCEP.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    PARP1 Protein, Human (sf9, His) purchased from MedChemExpress. Usage Cited in: Sci Adv. 2026 Jan 2;12(1):eady1681. [Abstract]

    Customer Validation
     Western blot analysis was conducted to measure the levels of FOXN3 in Parp1-/- A549 cells reintroduced with exogenous WT PARP1 or its E988A inactive mutant form.

  • PARP1 Protein, Human (sf9, His) Product Image
    1/16
  • Experimental Validation Results for PARP1 Protein, Human (sf9, His)
  • Experimental Validation Results for PARP1 Protein, Human (sf9, His)
  • PARP1 Protein, Human (sf9, His) Product Image

  • PARP1 Protein, Human (sf9, His) purchased from MedChemExpress. Usage Cited in: Sci Adv. 2026 Jan 2;12(1):eady1681. [Abstract]

    Western blot analysis was conducted to measure the levels of FOXN3 in Parp1-/- A549 cells reintroduced with exogenous WT PARP1 or its E988A inactive mutant form.