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ERO1L Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

ERO1L protein is an oxidoreductase that can effectively reoxidize P4HB/PDI in the endoplasmic reticulum and promote the formation of disulfide bonds. It transfers electrons to molecular oxygen, producing reactive oxygen species (ROS). ERO1L Protein, Human (HEK293, His) is the recombinant human-derived ERO1L protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • ERO1L Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

ERO1L protein is an oxidoreductase that can effectively reoxidize P4HB/PDI in the endoplasmic reticulum and promote the formation of disulfide bonds. It transfers electrons to molecular oxygen, producing reactive oxygen species (ROS). ERO1L Protein, Human (HEK293, His) is the recombinant human-derived ERO1L protein, expressed by HEK293 , with C-6*His labeled tag.

Background

ERO1L protein functions as an oxidoreductase, facilitating disulfide bond formation within the endoplasmic reticulum (ER). Its primary role involves efficiently reoxidizing P4HB/PDI, an enzyme crucial for catalyzing protein disulfide formation, enabling P4HB to engage in successive rounds of disulfide bond formation. Following the reoxidation of P4HB, ERO1L transfers electrons to molecular oxygen through its flavin adenine dinucleotide (FAD) cofactor, leading to the generation of reactive oxygen species (ROS) within the cell. This protein is essential for the proper folding of immunoglobulins and plays a significant role in ER stress-induced apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1, particularly in a CHOP-dependent manner. Additionally, ERO1L participates in the release of unfolded cholera toxin during V. cholerae infection, contributing to the retrotranslocation of the toxin.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q96HE7 (E24-H468)

  • Gene ID
    30001  [NCBI]
  • Molecular Construction
    • N-term
    • ERO1L (E24-H468)
      Accession # Q96HE7
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    ERO1A; Ero1alpha; Prev. ERO1L; ERO1-L-Alpha; Endoplasmic Reticulum Oxidoreductase Alpha; ERO1-L; ERO1-Like Protein Alpha; ERO1 (S. Cerevisiae)-Like; Oxidoreductin-1-L-Alpha; ERO1-Like (S. Cerevisiae); ERO1-Alpha; ERO1LA; Endoplasmic Reticulum Oxidoreducta

  • AA Sequence

    EEQPPETAAQRCFCQVSGYLDDCTCDVETIDRFNNYRLFPRLQKLLESDYFRYYKVNLKRPCPFWNDISQCGRRDCAVKPCQSDEVPDGIKSASYKYSEEANNLIEECEQAERLGAVDESLSEETQKAVLQWTKHDDSSDNFCEADDIQSPEAEYVDLLLNPERYTGYKGPDAWKIWNVIYEENCFKPQTIKRPLNPLASGQGTSEENTFYSWLEGLCVEKRAFYRLISGLHASINVHLSARYLLQETWLEKKWGHNITEFQQRFDGILTEGEGPRRLKNLYFLYLIELRALSKVLPFFERPDFQLFTGNKIQDEENKMLLLEILHEIKSFPLHFDENSFFAGDKKEAHKLKEDFRLHFRNISRIMDCVGCFKCRLWGKLQTQGLGTALKILFSEKLIANMPESGPSYEFHLTRQEIVSLFNAFGRISTSVKELENFRNLLQNIH

  • Predicted Molecular Mass

    53 kDa

  • Molecular Weight

    Approximately 71 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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