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ERO1L Protein, Human (HEK293, His)

Cat. No.: HY-P70893
COA Handling Instructions

ERO1L protein is an oxidoreductase that can effectively reoxidize P4HB/PDI in the endoplasmic reticulum and promote the formation of disulfide bonds. It transfers electrons to molecular oxygen, producing reactive oxygen species (ROS). ERO1L Protein, Human (HEK293, His) is the recombinant human-derived ERO1L protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ERO1L Protein, Human (HEK293, His) is 445 a.a., with molecular weight of ~71.0 kDa.

For research use only. We do not sell to patients.

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Description

ERO1L protein is an oxidoreductase that can effectively reoxidize P4HB/PDI in the endoplasmic reticulum and promote the formation of disulfide bonds. It transfers electrons to molecular oxygen, producing reactive oxygen species (ROS). ERO1L Protein, Human (HEK293, His) is the recombinant human-derived ERO1L protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ERO1L Protein, Human (HEK293, His) is 445 a.a., with molecular weight of ~71.0 kDa.

Background

ERO1L protein functions as an oxidoreductase, facilitating disulfide bond formation within the endoplasmic reticulum (ER). Its primary role involves efficiently reoxidizing P4HB/PDI, an enzyme crucial for catalyzing protein disulfide formation, enabling P4HB to engage in successive rounds of disulfide bond formation. Following the reoxidation of P4HB, ERO1L transfers electrons to molecular oxygen through its flavin adenine dinucleotide (FAD) cofactor, leading to the generation of reactive oxygen species (ROS) within the cell. This protein is essential for the proper folding of immunoglobulins and plays a significant role in ER stress-induced apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1, particularly in a CHOP-dependent manner. Additionally, ERO1L participates in the release of unfolded cholera toxin during V. cholerae infection, contributing to the retrotranslocation of the toxin.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q96HE7 (E24-H468)

Gene ID
Molecular Construction
N-term
ERO1L (E24-H468)
Accession # Q96HE7
6*His
C-term
Synonyms
ERO1-Like Protein Alpha; ERO1-L; ERO1-L-Alpha; Endoplasmic Oxidoreductin-1-Like Protein; Oxidoreductin-1-L-Alpha; ERO1L
AA Sequence

EEQPPETAAQRCFCQVSGYLDDCTCDVETIDRFNNYRLFPRLQKLLESDYFRYYKVNLKRPCPFWNDISQCGRRDCAVKPCQSDEVPDGIKSASYKYSEEANNLIEECEQAERLGAVDESLSEETQKAVLQWTKHDDSSDNFCEADDIQSPEAEYVDLLLNPERYTGYKGPDAWKIWNVIYEENCFKPQTIKRPLNPLASGQGTSEENTFYSWLEGLCVEKRAFYRLISGLHASINVHLSARYLLQETWLEKKWGHNITEFQQRFDGILTEGEGPRRLKNLYFLYLIELRALSKVLPFFERPDFQLFTGNKIQDEENKMLLLEILHEIKSFPLHFDENSFFAGDKKEAHKLKEDFRLHFRNISRIMDCVGCFKCRLWGKLQTQGLGTALKILFSEKLIANMPESGPSYEFHLTRQEIVSLFNAFGRISTSVKELENFRNLLQNIH

Molecular Weight

Approximately 71.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ERO1L Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ERO1L Protein, Human (HEK293, His)
Cat. No.:
HY-P70893
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