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PDIA3 Protein, Human (HEK293, His)

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The PDIA3 protein is a disulfide isomerase that catalyzes disulfide bonding processes and serves as a key protein folding partner. It is the core of the MHC I peptide loading complex and is essential for TAPBP folding. PDIA3 Protein, Human (HEK293, His) is the recombinant human-derived PDIA3, expressed by HEK293, with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The PDIA3 protein is a disulfide isomerase that catalyzes disulfide bonding processes and serves as a key protein folding partner. It is the core of the MHC I peptide loading complex and is essential for TAPBP folding. PDIA3 Protein, Human (HEK293, His) is the recombinant human-derived PDIA3, expressed by HEK293, with C-6*His labeled tag.

Background

PDIA3, a protein disulfide isomerase, plays a pivotal role in the intricate processes of catalyzing disulfide bond formation, isomerization, and reduction or oxidation in client proteins, functioning concurrently as a crucial protein folding chaperone. Within the major histocompatibility complex class I (MHC I) peptide loading complex, PDIA3 assumes a central position, serving as an indispensable folding chaperone for TAPBP. Collaboratively with TAPBP, PDIA3 facilitates the dynamic assembly of the MHC I complex, ensuring the seamless integration of high-affinity antigens within the endoplasmic reticulum. Consequently, PDIA3 emerges as a key player in orchestrating the presentation of antigens to cytotoxic T cells, thereby playing a critical role in adaptive immunity.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P30101 (S25-L505)

  • Gene ID
    2923  [NCBI]
  • Molecular Construction
    • N-term
    • PDIA3 (S25-L505)
      Accession # P30101
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    PDIA3; Disulfide Isomerase ER-60; Prev. GRP58; ER Protein 60; ERp57; HEL-S-93n; ERp60; Epididymis Secretory Sperm Binding Protein Li 93n; P58; Protein Disulfide Isomerase Family A, Member 3; Protein Disulfide-Isomerase A3; Epididymis Secretory Protein Li

  • AA Sequence

    SDVLELTDDNFESRISDTGSAGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL

  • Predicted Molecular Mass

    55.3 kDa

  • Molecular Weight

    Approximately 60 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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