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Periostin Protein, Human (758a.a, HEK293, His)

2 Cited Publications
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Based on 2 publication(s) in Google Scholar

Periostin protein, a multifaceted regulator, induces cell attachment and spreading, participates in cell adhesion, and enhances BMP1 incorporation into the fibronectin matrix. It forms a structural homodimer and interacts with BMP1 and fibronectin. This interplay positions Periostin as a key player in regulating cellular and tissue functions, including cell adhesion, tissue architecture, and extracellular matrix modulation. Periostin Protein, Human (758a.a, HEK293, His) is the recombinant human-derived Periostin protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Periostin Protein, Human (758a.a, HEK293, His)
  • Experimental Validation Results for Periostin Protein, Human (758a.a, HEK293, His)
  • Periostin Protein, Human (758a.a, HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Periostin protein, a multifaceted regulator, induces cell attachment and spreading, participates in cell adhesion, and enhances BMP1 incorporation into the fibronectin matrix. It forms a structural homodimer and interacts with BMP1 and fibronectin. This interplay positions Periostin as a key player in regulating cellular and tissue functions, including cell adhesion, tissue architecture, and extracellular matrix modulation. Periostin Protein, Human (758a.a, HEK293, His) is the recombinant human-derived Periostin protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Periostin protein emerges as a multifaceted regulator, demonstrating its capability to induce cell attachment and spreading while actively participating in cell adhesion. Additionally, Periostin enhances the incorporation of BMP1 into the fibronectin matrix within connective tissues, subsequently facilitating the proteolytic activation of lysyl oxidase LOX. This multifunctional protein exists as a homodimer, emphasizing its structural significance. Notably, Periostin engages in interactions with BMP1 and fibronectin, underscoring its involvement in pivotal molecular processes that contribute to cell adhesion, tissue architecture, and extracellular matrix modulation. The intricate interplay between Periostin and its binding partners positions it as a key player in the dynamic regulation of cellular and tissue functions.

Verified Bioactivity

1. Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 2.456 μg /mL, corresponding to a specific activity is 407.17 units/mg.
2. Measured by the ability of the immobilized protein to support the adhesion of ATDC5 cells. Immobilized Recombinant Human Periostin/OSF-2 at 10 µg/mL (100 µL/well) induces ≥50% cell.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 2.456 μg /mL, corresponding to a specific activity is 407.17 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q15063-2 (N22-Q779)

  • Gene ID
    10631  [NCBI]
  • Molecular Construction
    • N-term
    • Periostin (N22-Q779)
      Accession # Q15063-2
    • 6*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    POSTN; Osteoblast Specific Factor 2 (Fasciclin I-Like); Periostin; Periodontal Ligament-Specific Periostin; OSF-2; Osteoblast Specific Factor 2; PN; Osteoblast-Specific Factor 2; Periostin, Osteoblast Specific Factor; PDLPOSTN; OSF2

  • AA Sequence

    NNHYDKILAHSRIRGRDQGPNVCALQQILGTKKKYFSTCKNWYKKSICGQKTTVLYECCPGYMRMEGMKGCPAVLPIDHVYGTLGIVGATTTQRYSDASKLREEIEGKGSFTYFAPSNEAWDNLDSDIRRGLESNVNVELLNALHSHMINKRMLTKDLKNGMIIPSMYNNLGLFINHYPNGVVTVNCARIIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDDLSSFRAAAITSDILEALGRDGHFTLFAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSESIMGGAVFETLEGNTIEIGCDGDSITVNGIKMVNKKDIVTNNGVIHLIDQVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASALRPDGEYTLLAPVNNAFSDDTLSMDQRLLKLILQNHILKVKVGLNELYNGQILETIGGKQLRVFVYRTAVCIENSCMEKGSKQGRNGAIHIFREIIKPAEKSLHEKLKQDKRFSTFLSLLEAADLKELLTQPGDWTLFVPTNDAFKGMTSEEKEILIRDKNALQNIILYHLTPGVFIGKGFEPGVTNILKTTQGSKIFLKEVNDTLLVNELKSKESDIMTTNGVIHVVDKLLYPADTPVGNDQLLEILNKLIKYIQIKFVRGSTFKEIPVTVYKPIIKKYTKIIDGVPVEITEKETREERIITGPEIKYTRISTGGGETEETLKKLLQEEVTKVTKFIEGGDGHLFEDEEIKRLLQGDTPVRKLQANKKVQGSRRRLREGRSQ

  • Molecular Weight

    Approximately 85-90 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.0.
3.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.0, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 2.456 μg /mL, corresponding to a specific activity is 407.17 units/mg.

  • Periostin Protein, Human (758a.a, HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Periostin Protein, Human (758a.a, HEK293, His)
  • Experimental Validation Results for Periostin Protein, Human (758a.a, HEK293, His)
  • Periostin Protein, Human (758a.a, HEK293, His) Product Image