PF-4/CXCL4 Protein, Human (Biotinylated, His-Avi)

Wnt-10b protein is a signaling molecule that plays a key role in embryonic development and tissue homeostasis. It is involved in regulating cell proliferation, differentiation and migration. PF-4/CXCL4 Protein, Human (Biotinylated, His-Avi) is the recombinant human-derived PF-4/CXCL4 protein, expressed by E. coli , with C-Avi, N-His labeled tag.

The PF-4/CXCL4 Protein, a member of the CXC chemokine family, is encoded by this gene. Released from the alpha granules of activated platelets in the form of a homotetramer, this chemokine exhibits high affinity for heparin and plays a crucial role in platelet aggregation. Beyond its involvement in platelet function, PF-4/CXCL4 is chemotactic for various cell types and serves as an inhibitor of hematopoiesis, angiogenesis, and T-cell function. Additionally, it demonstrates antimicrobial activity against Plasmodium falciparum. The gene displays biased expression, with elevated levels in the bone marrow (RPKM 14.3), spleen (RPKM 2.7), and one other tissue, underscoring its potential significance in various physiological contexts across multiple organs.

Immobilized Human CCL5, His Tag at 5 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC₅₀ of 1.1 μg/mL determined by ELISA.

Human

E. coli

N-Avi;N-8*His

NP_002610.1 (E32-S101)

5196  [NCBI]

Full Length of Mature Protein

Biotin

EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES

Approximately 13 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 4 mM HCl, pH 3.0, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 4 mM HCl (pH 3.0).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.