1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Serine/Threonine Kinase Proteins
  4. PKC-nu
  5. PRKD3/nPKC nu Protein, Human (sf9, GST)

PRKD3/nPKC nu Protein, Human (sf9, GST)

Cat. No.: HY-P73699
Handling Instructions

PRKD3/nPKC nu protein acts as a mediator in the conversion of transient diacylglycerol (DAG) signals into sustained physiological effects, functioning downstream of PKC. Additionally, it plays a crucial role in conferring resistance to oxidative stress, demonstrating its involvement in cellular responses to environmental challenges. PRKD3/nPKC nu Protein, Human (sf9, GST) is the recombinant human-derived PRKD3/nPKC nu protein, expressed by Sf9 insect cells , with GST labeled tag. The total length of PRKD3/nPKC nu Protein, Human (sf9, GST) is 889 a.a., with molecular weight of ~126.7 kDa.

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Description

PRKD3/nPKC nu protein acts as a mediator in the conversion of transient diacylglycerol (DAG) signals into sustained physiological effects, functioning downstream of PKC. Additionally, it plays a crucial role in conferring resistance to oxidative stress, demonstrating its involvement in cellular responses to environmental challenges. PRKD3/nPKC nu Protein, Human (sf9, GST) is the recombinant human-derived PRKD3/nPKC nu protein, expressed by Sf9 insect cells , with GST labeled tag. The total length of PRKD3/nPKC nu Protein, Human (sf9, GST) is 889 a.a., with molecular weight of ~126.7 kDa.

Background

Protein kinase D3 (PRKD3), also known as novel protein kinase C nu (nPKC nu), serves a crucial role in cellular signaling by converting transient diacylglycerol (DAG) signals into sustained physiological effects, acting downstream of protein kinase C (PKC). This kinase's ability to modulate signaling cascades suggests its involvement in diverse cellular processes that rely on prolonged responses to diacylglycerol. Furthermore, PRKD3/nPKC nu has been implicated in conferring resistance to oxidative stress, indicating its potential role in cellular defense mechanisms against reactive oxygen species and related stressors (By similarity). The multifaceted functions of PRKD3/nPKC nu underscore its importance in signal transduction, suggesting its contribution to cellular adaptation and survival in response to various environmental challenges.

Species

Human

Source

Sf9 insect cells

Tag

GST

Accession

O94806 (S2-P890)

Gene ID
Synonyms
Serine/threonine-protein kinase D3; nPKC-nu; PRKD3; EPK2; PRKCN
Molecular Weight

Approximately 126.7 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

PRKD3/nPKC nu Protein, Human (sf9, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PRKD3/nPKC nu Protein, Human (sf9, GST)
Cat. No.:
HY-P73699
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