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  4. Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His)

Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His)

Cat. No.: HY-P7817A
COA Handling Instructions

Acid prostatic phosphatase (ACPP) is an enzyme that catalyzes the conversion of orthophosphate monoesters into alcohol and orthophosphate. ACPP is regulated by androgens and secreted by prostate epithelial cells. Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His) is the recombinant human-derived Prostatic acid phosphatase/ACPP protein, expressed by HEK293 , with C-His labeled tag. The total length of Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His) is 350 a.a., with molecular weight of 43-55 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Acid prostatic phosphatase (ACPP) is an enzyme that catalyzes the conversion of orthophosphate monoesters into alcohol and orthophosphate. ACPP is regulated by androgens and secreted by prostate epithelial cells. Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His) is the recombinant human-derived Prostatic acid phosphatase/ACPP protein, expressed by HEK293 , with C-His labeled tag. The total length of Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His) is 350 a.a., with molecular weight of 43-55 kDa.

Background

Prostatic acid phosphatase (ACPP) is an enzyme responsible for catalyzing the conversion of orthophosphoric monoester to alcohol and orthophosphate. Synthesized under androgen regulation, ACPP is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant has been identified, encoding a longer isoform with a transmembrane domain that localizes to the plasma membrane-endosomal-lysosomal pathway. The expression of ACPP is notably confined to the prostate, emphasizing its role as a marker associated with this specific tissue.

Biological Activity

1. Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >100,000 pmol/min/μg.
2. Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). which can be measured by absorbance at 410 nm. The specific activity is 4.264×107 pmol/min/µg, as measured under the described conditions.

Species

Human

Source

HEK293

Tag

C-His

Accession

NP_001127666.1 (K33-K382)

Gene ID

55  [NCBI]

Molecular Construction
N-term
ACPP (K33-K382)
Accession # NP_001127666.1
His
C-term
Synonyms
Prostatic Acid Phosphatase; PAP; 5'-Nucleotidase; 5'-NT, Ecto-5'-Nucleotidase; Thiamine Monophosphatase; TMPase; ACPP
AA Sequence

MRAAPLLLARAASLSLGFLFLLFFWLDRSVLAKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELVGPVIPQDWSTECMTTNSHQVLK

Molecular Weight

43-55 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE
Appearance

Lyophilized powder

Formulation

Lyophilized a 0.22 μm filtered solution of 20 mM PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His)
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HY-P7817A
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