Semaphorin-4D/SEMA4D Protein, Human (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
Semaphorin-4D/SEMA4D protein, a receptor for PLXNB1 and PLXNB2, regulates cell-cell signaling. It promotes inhibitory synapse development via PLXNB1, modulates neurite arborization through RHOA activation, and enhances viability of B-cells. SEMA4D induces endothelial cell migration via PTK2B/PYK2, SRC, and PI3K-AKT pathway. It forms homodimers and interacts with PLXNB2 and PLXNB1. Semaphorin-4D/SEMA4D Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Semaphorin-4D/SEMA4D protein, a receptor for PLXNB1 and PLXNB2, regulates cell-cell signaling. It promotes inhibitory synapse development via PLXNB1, modulates neurite arborization through RHOA activation, and enhances viability of B-cells. SEMA4D induces endothelial cell migration via PTK2B/PYK2, SRC, and PI3K-AKT pathway. It forms homodimers and interacts with PLXNB2 and PLXNB1. Semaphorin-4D/SEMA4D Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
Background
Semaphorin-4D/SEMA4D Protein, a cell surface receptor for PLXNB1 and PLXNB2, plays a crucial role in cell-cell signaling. It is involved in regulating GABAergic synapse development and promotes the development of inhibitory synapses in a PLXNB1-dependent manner. By activating PLXNB1 and interacting with it, SEMA4D modulates the complexity and arborization of developing neurites in hippocampal neurons, mediating the activation of RHOA. Additionally, SEMA4D promotes the migration of cerebellar granule cells and induces B-cells to aggregate, enhancing their viability in vitro. In the immune system, SEMA4D plays a role in inducing endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway. It forms homodimers and interacts with PLXNB2, as well as interacts with PLXNB1.
Verified Bioactivity
1.Measured by its binding ability in a functional ELISA. When immobilized Anti-Semaphorin 4D Antibody hFc at 0.5 μg/mL (100μL/Well), can bind Human Semaphorin 4D His Tag and the EC50 is 55.2 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Human Semaphorin 4D,His Tag at 1 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Anti-Semaphorin 4D Antibody, hFc Tag with the EC50 of 1.0-10.0 ng/mL determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-8*His
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Accession
Q92854-1 (M22-R734)
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Molecular Construction
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N-term
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SEMA4D (M22-R734)
Accession # Q92854-1 -
8*His-Avi
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C-term
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Protein Length
Extracellular Domain
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Conjugation
Biotin
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Synonyms
SEMA4D; BB18; Prev. C9orf164; A8; Prev. SEMAJ; Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) And Short Cytoplasmic Domain, 4D; CD100; Chromosome 9 Open Reading Frame 164; Semaphorin-4D; CD100 Antigen; Coll-4; M-Sema G; Sema Domain, Im
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AA Sequence
MAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNR
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Predicted Molecular Mass
82.1 kDa
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Molecular Weight
Approximately 115-140 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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