1. Recombinant Proteins
  2. CD Antigens Biotinylated Proteins
  3. NK Cell CD Proteins
  4. CD100/Semaphorin-4D
  5. Semaphorin-4D/SEMA4D Protein, Human (Biotinylated, HEK293, His-Avi)

Semaphorin-4D/SEMA4D Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78202
Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
20 μg $245 In-stock
Estimated Time of Arrival: December 31
50 μg   Get quote  
100 μg   Get quote  

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity
Measured by its binding ability in a functional ELISA. When immobilized Anti-Semaphorin 4D Antibody hFc at 0.5 μg/mL (100μL/Well), can bind Human Semaphorin 4D His Tag and the EC50 is 55.5 ng/mL.
Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

Q92854 (M22-R734)

Gene ID
Synonyms
Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4
Molecular Weight

100-130 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Semaphorin-4D/SEMA4D Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Semaphorin-4D/SEMA4D Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P78202
Quantity:
MCE Japan Authorized Agent: