Semaphorin-4D/SEMA4D Protein, Human (Biotinylated, HEK293, His-Avi)

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
20 μg	$245	In-stock	Estimated Time of Arrival: December 31
50 μg	$540	In-stock	Estimated Time of Arrival: December 31
100 μg		Get quote
250 μg		Get quote

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Biological Activity	Measured by its binding ability in a functional ELISA. When immobilized Anti-Semaphorin 4D Antibody hFc at 0.5 μg/mL (100μL/Well), can bind Human Semaphorin 4D His Tag and the EC₅₀ is 55.2 ng/mL.
Species	Human
Source	HEK293
Tag	C-Avi;C-His
Accession	Q92854 (M22-R734)
Gene ID	10507 [NCBI]
Synonyms	Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4
Molecular Weight	100-130 kDa
Purity	Greater than 95% as determined by Tris-Bis PAGE.
Appearance	Lyophilized powder.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.
Endotoxin Level	<1 EU/μg, determined by LAL method.
Reconstitution	It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.
Storage & Stability	Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping	Room temperature in continental US; may vary elsewhere.
Documentation	Select Batch: Data Sheet (232 KB) SDS (251 KB) COA (220 KB) Handling Instructions (2659 KB)

Semaphorin-4D/SEMA4D Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Specific Activity (Unit/mg) = 10⁶ ÷ Biological Activity (ED₅₀)

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