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Serpin A3 Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

Serpin A3 belongs to the serine protease inhibitor family and can interact with DNAJC1. Serpin A3 can inhibit neutrophil cathepsin G and mast cell chymase and participate in the conversion of ACE1 to ACE2. Serpin A3 Protein, Human (HEK293, His) is the recombinant human-derived Serpin A3 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Serpin A3 Protein, Human (HEK293, His)
  • Serpin A3 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Serpin A3 belongs to the serine protease inhibitor family and can interact with DNAJC1. Serpin A3 can inhibit neutrophil cathepsin G and mast cell chymase and participate in the conversion of ACE1 to ACE2. Serpin A3 Protein, Human (HEK293, His) is the recombinant human-derived Serpin A3 protein, expressed by HEK293 , with C-His labeled tag.

Background

Serpin A3, also known as alpha-1-antichymotrypsin (ACT or AACT), also known as cytostatic 24/25 protein, is a member of the serine protease inhibitor (serpin) family. Serpin A3 has been found to have inhibitory activity against neutrophil cathepsin G and mast cell tryptase, two enzymes involved in the conversion of angiotensin ACE1 to biologically active ACE2. In addition, Serpin A3 can interact with DNAJC1.

Verified Bioactivity

Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2 (HY-P2185A). The IC50 is <15 nM.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    NP_001076.2 (N26-A423)

  • Gene ID
    12  [NCBI]
  • Molecular Construction
    • N-term
    • Serpin A3 (N26-A423)
      Accession # NP_001076.2
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    SERPINA3; Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3, Isoform CRA_a; Prev. AACT; Growth-Inhibiting Protein 24; ACT; Growth-Inhibiting Protein 25; Alpha-1-Antichymotrypsin; Alpha-1 Antichymotrypsin; Serpin Peptidase

  • AA Sequence

    NSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYTGNASALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQA

  • Molecular Weight

    Approximately 45 kDa & 55-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 25 mM HEPES, 0.15M NaCl, pH 7.8.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Serpin A3 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Serpin A3 Protein, Human (HEK293, His)
  • Serpin A3 Protein, Human (HEK293, His) Product Image