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Siglec-1/CD169 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

Siglec-1/CD169 is a macrophage-specific adhesion molecule that mediates sialic acid-dependent binding to immune cells, limiting bacterial dissemination and enhancing phagocytosis. In addition to bacterial interactions, it facilitates the uptake of enveloped viruses and promotes macrophage-to-T cell dissemination. Siglec-1/CD169 Protein, Human (HEK293, His) is the recombinant human-derived Siglec-1/CD169 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Siglec-1/CD169 Protein, Human (HEK293, His)
  • Siglec-1/CD169 Protein, Human (HEK293, His) Product Image
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Siglec-1/CD169 is a macrophage-specific adhesion molecule that mediates sialic acid-dependent binding to immune cells, limiting bacterial dissemination and enhancing phagocytosis. In addition to bacterial interactions, it facilitates the uptake of enveloped viruses and promotes macrophage-to-T cell dissemination. Siglec-1/CD169 Protein, Human (HEK293, His) is the recombinant human-derived Siglec-1/CD169 protein, expressed by HEK293 , with C-His labeled tag.

Background

Siglec-1/CD169 emerges as a macrophage-restricted adhesion molecule with a pivotal role in mediating sialic acid-dependent binding to various immune cells, including granulocytes, monocytes, natural killer cells, B-cells, and CD8 T-cells. Its significance lies in limiting bacterial dissemination by engaging sialylated bacteria, thereby promoting effective phagocytosis and antigen presentation crucial for the adaptive immune response. Beyond bacterial interactions, Siglec-1 is implicated in the uptake of enveloped viruses through sialic acid recognition, leading to the formation of intracellular compartments filled with virions (VCCs). This facilitates macrophage-to-T-cell transmission of viruses, including HIV-1 or SARS-CoV-2. Acting as an endocytic receptor, Siglec-1 employs clathrin-dependent endocytosis, exhibiting a preference for alpha-2,3-linked sialic acid. Notably, it binds to SPN/CD43 on T-cells and may play a role in hemopoiesis. Additionally, Siglec-1 contributes to the inhibition of antiviral innate immunity by promoting TBK1 degradation via TYROBP and TRIM27-mediated ubiquitination. In the context of microbial infection, it facilitates viral cytoplasmic entry into activated dendritic cells by recognizing sialylated gangliosides present on the viral membrane.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of THP-1.The ED50 for this effect is 2.863 µg/mL, corresponding to a specific activity is 349.284 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q9BZZ2-1 (S20-Q1641)

  • Gene ID
    6614  [NCBI]
  • Molecular Construction
    • N-term
    • CD169 (S20-Q1641)
      Accession # Q9BZZ2-1
    • 10*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    SIGLEC1; DJ1009E24.1; Prev. SN; FLJ00055; Sialoadhesin; FLJ00073; SIGLEC-1; FLJ32150; CD169; Sialic Acid-Binding Immunoglobulin-Like Lectin 1; Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin; CD169 Antigen; Sialic Acid-Binding Ig-Like Lectin 1; Siglec-

  • AA Sequence

    SWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKYAPKGVKILLSPSGRNILPGELVTLTCQVNSSYPAVSSIKWLKDGVRLQTKTGVLHLPQAAWSDAGVYTCQAENGVGSLVSPPISLHIFMAEVQVSPAGPILENQTVTLVCNTPNEAPSDLRYSWYKNHVLLEDAHSHTLRLHLATRADTGFYFCEVQNVHGSERSGPVSVVVNHPPLTPVLTAFLETQAGLVGILHCSVVSEPLATLVLSHGGHILASTSGDSDHSPRFSGTSGPNSLRLEIRDLEETDSGEYKCSATNSLGNATSTLDFHANAARLLISPAAEVVEGQAVTLSCRSGLSPTPDARFSWYLNGALLHEGPGSSLLLPAASSTDAGSYHCRARDGHSASGPSSPAVLTVLYPPRQPTFTTRLDLDAAGAGAGRRGLLLCRVDSDPPARLQLLHKDRVVATSLPSGGGCSTCGGCSPRMKVTKAPNLLRVEIHNPLLEEEGLYLCEASNALGNASTSATFNGQATVLAIAPSHTLQEGTEANLTCNVSREAAGSPANFSWFRNGVLWAQGPLETVTLLPVARTDAALYACRILTEAGAQLSTPVLLSVLYPPDRPKLSALLDMGQGHMALFICTVDSRPLALLALFHGEHLLATSLGPQVPSHGRFQAKAEANSLKLEVRELGLGDSGSYRCEATNVLGSSNTSLFFQVRGAWVQVSPSPELQEGQAVVLSCQVHTGVPEGTSYRWYRDGQPLQESTSATLRFAAITLTQAGAYHCQAQAPGSATTSLAAPISLHVSYAPRHVTLTTLMDTGPGRLGLLLCRVDSDPPAQLRLLHGDRLVASTLQGVGGPEGSSPRLHVAVAPNTLRLEIHGAMLEDEGVYICEASNTLGQASASADFDAQAVNVQVWPGATVREGQLVNLTCLVWTTHPAQLTYTWYQDGQQRLDAHSIPLPNVTVRDATSYRCGVGPPGRAPRLSRPITLDVLYAPRNLRLTYLLESHGGQLALVLCTVDSRPPAQLALSHAGRLLASSTAASVPNTLRLELRGPQPRDEGFYSCSARSPLGQANTSLELRLEGVRVILAPEAAVPEGAPITVTCADPAAHAPTLYTWYHNGRWLQEGPAASLSFLVATRAHAGAYSCQAQDAQGTRSSRPAALQVLYAPQDAVLSSFRDSRARSMAVIQCTVDSEPPAELALSHDGKVLATSSGVHSLASGTGHVQVARNALRLQVQDVPAGDDTYVCTAQNLLGSISTIGRLQVEGARVVAEPGLDVPEGAALNLSCRLLGGPGPVGNSTFAWFWNDRRLHAEPVPTLAFTHVARAQAGMYHCLAELPTGAAASAPVMLRVLYPPKTPTMMVFVEPEGGLRGILDCRVDSEPLASLTLHLGSRLVASSQPQGAPAEPHIHVLASPNALRVDIEALRPSDQGEYICSASNVLGSASTSTYFGVRALHRLHQFQQ

  • Predicted Molecular Mass

    174.6 kDa

  • Molecular Weight

    Approximately 130-180 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized a 0.22 μm filtered solution of 20 mM MES,150 mM NaCl, pH 5.5, 10% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Siglec-1/CD169 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Siglec-1/CD169 Protein, Human (HEK293, His)
  • Siglec-1/CD169 Protein, Human (HEK293, His) Product Image