STK40 Protein, Human (sf9, His-GST)

The STK40 protein functions as a potential negative regulator, inhibiting NF-kappa-B and p53-mediated gene transcription. Its regulatory role implies fine-tuning of signaling pathways, indicating regulation of gene expression. STK40 Protein, Human (sf9, His-GST) is the recombinant human-derived STK40 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

STK40 Protein emerges as a potential negative regulator, exerting influence over NF-kappa-B and p53-mediated gene transcription. Its regulatory role implies an inhibitory function, suggesting involvement in fine-tuning the intricate signaling pathways associated with NF-kappa-B and p53. By acting as a negative regulator, STK40 may modulate the expression of genes under the control of these transcription factors, influencing cellular responses to various stimuli. Elucidating the specific mechanisms through which STK40 exerts its regulatory effects on NF-kappa-B and p53-mediated transcription could provide valuable insights into its role in cellular homeostasis and response to stressors. Further exploration of STK40's interactions and downstream effects may contribute to a comprehensive understanding of its function in the intricate network of transcriptional regulation.

The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His;N-GST

Q8N2I9-1 (M1-K435)

83931  [NCBI]

Full Length of Isoform-1

MKRRASDRGAGETSARAKALGSGISGNNAKRAGPFILGPRLGNSPVPSIVQCLARKDGTDDFYQLKILTLEERGDQGIESQEERQGKMLLHTEYSLLSLLHTQDGVVHHHGLFQDRTCEIVEDTESSRMVKKMKKRICLVLDCLCAHDFSDKTADLINLQHYVIKEKRLSERETVVIFYDVVRVVEALHQKNIVHRDLKLGNMVLNKRTHRITITNFCLGKHLVSEGDLLKDQRGSPAYISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPEDGRVSENTVCLIRKLLVLDPQQRLAAADVLEALSAIIASWQSLSSLSGPLQVVPDIDDQMSNADSSQEAKVTEECSQYEFENYMRQQLLLAEEKSSIHDARSWVPKRQFGSAPPVRRLGHDAQPMTSLDTAILAQRYLRK

Approximately 85 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, 10% glycerol, 1 mM TCEP, 0.5 M Urea, 0.5 mM GSH, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.