TLK1 Protein, Human (sf9, His-GST)

The TLK1 protein is an integral part of cellular processes and undergoes rapid, transient phosphorylation-induced inhibition during S phase in response to DNA double-strand breaks. This ATM pathway-dependent checkpoint mechanism plays a crucial role in chromatin assembly. TLK1 Protein, Human (sf9, His-GST) is the recombinant human-derived TLK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

TLK1, a protein intricately involved in cellular processes, experiences rapid and transient inhibition through phosphorylation upon the occurrence of DNA double-stranded breaks during S-phase. This regulatory mechanism is checkpoint-dependent, involving the ATM pathway, and plays a crucial role in orchestrating processes associated with chromatin assembly. Notably, isoform 3 of TLK1 exhibits the capability to phosphorylate and enhance the stability of the t-SNARE SNAP23, thereby promoting its assembly with syntaxin. Additionally, this isoform serves a protective role against ionizing radiation by facilitating the repair of DNA double-strand breaks. In vitro, TLK1 phosphorylates histone H3 at 'Ser-10,' further emphasizing its involvement in chromatin dynamics and cellular response to DNA damage.

Human

Sf9 insect cells

N-His;N-GST

Q9UKI8 (M1-Y766)

9874  [NCBI]

Full Length

MSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRPREGAMDELHSLDPRRQELLEARFTGVASGSTGSTGSCSVGAKASTNNESSNHSFGSLGSLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIEKSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY

Approximately 125 kDa, based on SDS-PAGE under reducing conditions.

≥ 85%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.