TLK1 Protein, Human (sf9, His-GST)
The TLK1 protein is an integral part of cellular processes and undergoes rapid, transient phosphorylation-induced inhibition during S phase in response to DNA double-strand breaks. This ATM pathway-dependent checkpoint mechanism plays a crucial role in chromatin assembly. TLK1 Protein, Human (sf9, His-GST) is the recombinant human-derived TLK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The TLK1 protein is an integral part of cellular processes and undergoes rapid, transient phosphorylation-induced inhibition during S phase in response to DNA double-strand breaks. This ATM pathway-dependent checkpoint mechanism plays a crucial role in chromatin assembly. TLK1 Protein, Human (sf9, His-GST) is the recombinant human-derived TLK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
TLK1, a protein intricately involved in cellular processes, experiences rapid and transient inhibition through phosphorylation upon the occurrence of DNA double-stranded breaks during S-phase. This regulatory mechanism is checkpoint-dependent, involving the ATM pathway, and plays a crucial role in orchestrating processes associated with chromatin assembly. Notably, isoform 3 of TLK1 exhibits the capability to phosphorylate and enhance the stability of the t-SNARE SNAP23, thereby promoting its assembly with syntaxin. Additionally, this isoform serves a protective role against ionizing radiation by facilitating the repair of DNA double-strand breaks. In vitro, TLK1 phosphorylates histone H3 at 'Ser-10,' further emphasizing its involvement in chromatin dynamics and cellular response to DNA damage.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
Q9UKI8 (M1-Y766)
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Molecular Construction
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N-term
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His-GST
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TLK1 (M1-Y766)
Accession # Q9UKI8 -
C-term
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Protein Length
Full Length
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Synonyms
TLK1; Non-Specific Serine/Threonine Protein Kinase; Tousled Like Kinase 1; Tousled-Like Kinase 1, Isoform CRA_b; KIAA0137; Serine Threonine Protein Kinase; PKU-BETA; Uncharacterized Protein TLK1; Serine/Threonine-Protein Kinase Tousled-Like 1; SNARE Prote
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AA Sequence
MSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRPREGAMDELHSLDPRRQELLEARFTGVASGSTGSTGSCSVGAKASTNNESSNHSFGSLGSLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIEKSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY
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Predicted Molecular Mass
114 kDa
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Molecular Weight
Approximately 125 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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