TPST1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
TPST1 (tyrosyl protein sulfotransferase 1) catalyzes the O-sulfation of tyrosine residues located within the acidic motifs of polypeptides, utilizing 3'-phosphoadenylate sulfate (PAPS) as a co-substrate. This enzymatic activity plays a crucial role in post-translational modification processes, especially sulfation, which contributes to the structural and functional diversity of proteins. TPST1 Protein, Human (HEK293, His) is the recombinant human-derived TPST1, expressed by HEK293, with N-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
TPST1 (tyrosyl protein sulfotransferase 1) catalyzes the O-sulfation of tyrosine residues located within the acidic motifs of polypeptides, utilizing 3'-phosphoadenylate sulfate (PAPS) as a co-substrate. This enzymatic activity plays a crucial role in post-translational modification processes, especially sulfation, which contributes to the structural and functional diversity of proteins. TPST1 Protein, Human (HEK293, His) is the recombinant human-derived TPST1, expressed by HEK293, with N-His labeled tag.
Tyrosylprotein sulfotransferase 1 (TPST1) is a key enzyme that catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, utilizing 3'-phosphoadenylyl sulfate (PAPS) as a cosubstrate. This enzymatic process involves the transfer of a sulfate group to tyrosine residues, modulating the structure and function of proteins with acidic motifs. TPST1-mediated tyrosine sulfation is crucial for the post-translational modification of proteins involved in various biological processes, including cell signaling and receptor activation. By participating in the sulfation of tyrosine residues, TPST1 contributes to the diversity of protein modifications, influencing the interactions and activities of sulfated proteins in cellular pathways. This enzyme's role in tyrosine sulfation underscores its importance in the regulation of protein function and cellular signaling events.
Technical Parameters
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Species Human
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Source HEK293
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Tag N-His
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Accession
O60507 (Q26-E370)
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Molecular Construction
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N-term
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His
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TPST1 (Q26-E370)
Accession # O60507 -
C-term
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Protein Length
Lumenal Domain
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Synonyms
TPST1; Transport And Golgi Organization 13 Homolog A (Drosophila); Tyrosylprotein Sulfotransferase 1; Transport And Golgi Organization 13 Homolog A; TANGO13A; Protein-Tyrosine Sulfotransferase; Protein-Tyrosine Sulfotransferase 1; Uncharacterized Protein
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AA Sequence
QHAMECHHRIEERSQPVKLESTRTTVRTGLDLKANKTFAYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEIIVKHGEPAPYLCNKDPFALKSLTYLSRLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTLLKFLQIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKIIENTRRVYKGEFQLPDFLKEKPQTEQVE
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Predicted Molecular Mass
39.5 kDa
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Molecular Weight
Approximately 43-57 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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