1. Recombinant Proteins
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E2 Enzymes
  5. Ubiquitin Conjugating Enzyme E2 G1
  6. UBE2G1 Protein, Human

UBE2G1 Protein, Human

Cat. No.: HY-P77271
COA Handling Instructions

UBE2G1 Protein, a crucial ubiquitin-proteasome system player, acts as an E2 ubiquitin-conjugating enzyme, facilitating ubiquitin attachment to diverse protein substrates. In vitro, UBE2G1 demonstrates catalytic competence in 'Lys-48' and 'Lys-63'-linked polyubiquitination reactions. This multifaceted enzyme is implicated in potential roles, including the degradation of muscle-specific proteins, emphasizing its involvement in cellular processes related to protein turnover. Additionally, UBE2G1 mediates the polyubiquitination of CYP3A4, a cytochrome P450 enzyme, underscoring its significance in regulating specific cellular pathways. UBE2G1 Protein, Human is the recombinant human-derived UBE2G1 protein, expressed by E. coli, with tag free. The total length of UBE2G1 Protein, Human is 170 a.a., with molecular weight of ~19.5 kDa.

For research use only. We do not sell to patients.

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Description

UBE2G1 Protein, a crucial ubiquitin-proteasome system player, acts as an E2 ubiquitin-conjugating enzyme, facilitating ubiquitin attachment to diverse protein substrates. In vitro, UBE2G1 demonstrates catalytic competence in 'Lys-48' and 'Lys-63'-linked polyubiquitination reactions. This multifaceted enzyme is implicated in potential roles, including the degradation of muscle-specific proteins, emphasizing its involvement in cellular processes related to protein turnover. Additionally, UBE2G1 mediates the polyubiquitination of CYP3A4, a cytochrome P450 enzyme, underscoring its significance in regulating specific cellular pathways. UBE2G1 Protein, Human is the recombinant human-derived UBE2G1 protein, expressed by E. coli, with tag free. The total length of UBE2G1 Protein, Human is 170 a.a., with molecular weight of ~19.5 kDa.

Background

UBE2G1, an essential player in the ubiquitin-proteasome system, serves as an E2 ubiquitin-conjugating enzyme, facilitating the covalent attachment of ubiquitin to diverse protein substrates. In vitro, UBE2G1 showcases its catalytic competence by catalyzing both 'Lys-48'- and 'Lys-63'-linked polyubiquitination reactions. This multifaceted enzyme is implicated in potential roles, including the degradation of muscle-specific proteins, highlighting its involvement in cellular processes related to protein turnover. Additionally, UBE2G1 demonstrates its functional relevance by mediating the polyubiquitination of CYP3A4, a cytochrome P450 enzyme involved in the metabolism of various substrates, underscoring its significance in regulating specific cellular pathways.

Biological Activity

Under ATP and E1 conditions, E2 covalently binds with ubiquitin molecules to form thioester complexes E2~Ub.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

P62253 (M1-E170)

Gene ID
Molecular Construction
N-term
UBE2G1 (M1-E170)
Accession # P62253
C-term
Synonyms
Ubiquitin-conjugating enzyme E2 G1; E217K; UBC7; UBE2G1; UBE2G
AA Sequence

MTELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGEDKYGYEKPEERWLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARCVRKSQETAFE

Molecular Weight

Approximately 19.5 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
UBE2G1 Protein, Human
Cat. No.:
HY-P77271
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