VNN1/Vanin-1 Protein, Mouse (HEK293, His)

Vanin-1 (VNN1), an amidohydrolase, specifically hydrolyzes one carboamide linkage in D-pantetheine. This enzymatic function is crucial for recycling pantothenic acid (vitamin B5), releasing cysteamine. VNN1's breakdown of D-pantetheine contributes to maintaining sufficient pantothenic acid levels, vital for various biochemical pathways. This enzymatic action emphasizes VNN1's role in regulating vitamin B5 homeostasis and cysteamine release, highlighting its significance in cellular metabolism. VNN1/Vanin-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived VNN1/Vanin-1 protein, expressed by HEK293, with C-His labeled tag. The total length of VNN1/Vanin-1 Protein, Mouse (HEK293, His) is 465 a.a., with molecular weight of 55-70 kDa.

Vanin-1 (VNN1) is an amidohydrolase with the specific function of hydrolyzing one of the carboamide linkages in D-pantetheine. This enzymatic activity plays a crucial role in the recycling of pantothenic acid (vitamin B5), ultimately releasing cysteamine. By facilitating the breakdown of D-pantetheine, VNN1 contributes to the metabolic processes involved in maintaining adequate levels of pantothenic acid, a vital component in various biochemical pathways. This enzymatic action underscores the significance of VNN1 in the regulation of vitamin B5 homeostasis and the release of cysteamine, highlighting its role in cellular metabolism.

Mouse

HEK293

C-His

Q9Z0K8 (L24-N488)

22361  [NCBI]

55-70 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.