Vanin-1 Protein, Human (HEK293, His)

Vanin-1 Proteinas, as an amidohydrolase, specifically targets D-pantetheine's carboamide linkage, crucial for recycling pantothenic acid and releasing cysteamine. Its enzymatic breakdown of D-pantetheine underscores its significance in vitamin B5 recycling and cysteamine liberation metabolic processes. Vanin-1 Protein, Human (HEK293, His) is the recombinant human-derived Vanin-1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Vanin-1 Protein, Human (HEK293, His) is 469 a.a., with molecular weight of ~77.6 kDa.

Vanin-1 is an amidohydrolase with a specific enzymatic function, targeting one of the carboamide linkages in D-pantetheine. This activity is instrumental in the recycling of pantothenic acid (vitamin B5) and the concomitant release of cysteamine. Vanin-1's role in the enzymatic breakdown of D-pantetheine underscores its significance in the metabolic processes associated with vitamin B5 recycling and cysteamine liberation.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

O95497 (Q22-S490)

8876  [NCBI]

QDTFTAAVYEHAAILPNATLTPVSREEALALMNRNLDILEGAITSAADQGAHIIVTPEDAIYGWNFNRDSLYPYLEDIPDPEVNWIPCNNRNRFGQTPVQERLSCLAKNNSIYVVANIGDKKPCDTSDPQCPPDGRYQYNTDVVFDSQGKLVARYHKQNLFMGENQFNVPKEPEIVTFNTTFGSFGIFTCFDILFHDPAVTLVKDFHVDTIVFPTAWMNVLPHLSAVEFHSAWAMGMRVNFLASNIHYPSKKMTGSGIYAPNSSRAFHYDMKTEEGKLLLSQLDSHPSHSAVVNWTSYASSIEALSSGNKEFKGTVFFDEFTFVKLTGVAGNYTVCQKDLCCHLSYKMSENIPNEVYALGAFDGLHTVEGRYYLQICTLLKCKTTNLNTCGDSAETASTRFEMFSLSGTFGTQYVFPEVLLSENQLAPGEFQVSTDGRLFSLKPTSGPVLTVTLFGRLYEKDWASNASS

Approximately 77.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.