Copy Link Print Information

WISP1/CCN4 Protein, Mouse (HEK293, His)

Customer Review

Based on 1 Customer Validation

WISP1/CCN4 Protein, a member of the CCN family, is involved in cellular processes such as cell proliferation, migration, and differentiation.It is implicated in various diseases, including cancer and fibrosis.The potential therapeutic targeting of WISP1/CCN4 Protein and its role in disease pathogenesis make it a subject of interest in biomedical research.WISP1/CCN4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived WISP1/CCN4 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for WISP1/CCN4 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for WISP1/CCN4 Protein, Mouse (HEK293, His)
  • WISP1/CCN4 Protein, Mouse (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

WISP1/CCN4 Protein, a member of the CCN family, is involved in cellular processes such as cell proliferation, migration, and differentiation.It is implicated in various diseases, including cancer and fibrosis.The potential therapeutic targeting of WISP1/CCN4 Protein and its role in disease pathogenesis make it a subject of interest in biomedical research.WISP1/CCN4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived WISP1/CCN4 protein, expressed by HEK293 , with C-His labeled tag.

Background

WISP1/CCN4 protein acts as a downstream regulator in the Wnt/Frizzled-signaling pathway and is involved in cell survival. It shows adherence to skin and melanoma fibroblasts, potentially through binding to proteoglycans like decorin and biglycan. Additionally, WISP1/CCN4 protein has demonstrated the ability to suppress tumor growth in vivo.

Verified Bioactivity

Measured by its ability to inhibit the cell growth of DU145 human prostate cancer cells. The ED50 for this effect is 0.5-3.0 µg/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    O54775 (T23-N367)

  • Gene ID
    22402  [NCBI]
  • Molecular Construction
    • N-term
    • WISP1 (T23-N367)
      Accession # O54775
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CCN4; CCN Family Member 4; Prev. WISP1; WNT1 Induced Secreted Protein 1; Prev. WISP1-OT1; WISP1 Overlapping Transcript 1; Prev. WISP1-UT1; Wnt-1-Induced Secreted Protein; WISP-1; WISP1tc; WISP1 Overlapping Transcript 1 (Non-Protein Coding); WISP1c; WNT1 Inducible Signaling Pathway Protein 1; WISP1i; WISP1 3'UTR-Associated RNA 1; Cellular Communication Network Factor 4; WNT1-Inducible-Signaling Pathway Protein 1

  • AA Sequence

    TALSPTPTTMTFTPAPLEETTTRPEFCKWPCECPQSPPRCPLGVSLITDGCECCKICAQQLGDNCTEAAICDPHRGLYCDYSGDRPRYAIGVCAQVVGVGCVLDGVRYTNGESFQPNCRYNCTCIDGTVGCTPLCLSPRPPRLWCRQPRHVRVPGQCCEQWVCDDDARRPRQTALLDTRAFAASGAVEQRYENCIAYTSPWSPCSTTCGLGISTRISNVNARCWPEQESRLCNLRPCDVDIQLHIKAGKKCLAVYQPEEATNFTLAGCVSTRTYRPKYCGVCTDNRCCIPYKSKTISVDFQCPEGPGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFEEIAN

  • Molecular Weight

    Approximately 64-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit the cell growth of DU145 human prostate cancer cells. The ED50 for this effect is 0.9133 µg/mL, corresponding to a specific activity is 1094.930 U/mg.

  • WISP1/CCN4 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for WISP1/CCN4 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for WISP1/CCN4 Protein, Mouse (HEK293, His)
  • WISP1/CCN4 Protein, Mouse (HEK293, His) Product Image