WISP1/CCN4 Protein, Mouse (HEK293, His)

WISP1/CCN4 Protein, a member of the CCN family, is involved in cellular processes such as cell proliferation, migration, and differentiation. It is implicated in various diseases, including cancer and fibrosis. The potential therapeutic targeting of WISP1/CCN4 Protein and its role in disease pathogenesis make it a subject of interest in biomedical research. WISP1/CCN4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived WISP1/CCN4 protein, expressed by HEK293 , with C-His labeled tag. The total length of WISP1/CCN4 Protein, Mouse (HEK293, His) is 345 a.a., with molecular weight of 55-75 kDa.

WISP1/CCN4 protein acts as a downstream regulator in the Wnt/Frizzled-signaling pathway and is involved in cell survival. It shows adherence to skin and melanoma fibroblasts, potentially through binding to proteoglycans like decorin and biglycan. Additionally, WISP1/CCN4 protein has demonstrated the ability to suppress tumor growth in vivo.

Measured by its ability to inhibit the cell growth of DU145 human prostate cancer cells. The ED₅₀ for this effect is 0.9133 µg/mL, corresponding to a specific activity is 1094.930 U/mg.

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  Measured by its ability to inhibit the cell growth of DU145 human prostate cancer cells. The ED₅₀ for this effect is 0.9133 µg/mL, corresponding to a specific activity is 1094.930 U/mg.

Mouse

HEK293

C-His

O54775 (T23-N367)

22402  [NCBI]

TALSPTPTTMTFTPAPLEETTTRPEFCKWPCECPQSPPRCPLGVSLITDGCECCKICAQQLGDNCTEAAICDPHRGLYCDYSGDRPRYAIGVCAQVVGVGCVLDGVRYTNGESFQPNCRYNCTCIDGTVGCTPLCLSPRPPRLWCRQPRHVRVPGQCCEQWVCDDDARRPRQTALLDTRAFAASGAVEQRYENCIAYTSPWSPCSTTCGLGISTRISNVNARCWPEQESRLCNLRPCDVDIQLHIKAGKKCLAVYQPEEATNFTLAGCVSTRTYRPKYCGVCTDNRCCIPYKSKTISVDFQCPEGPGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFEEIAN

55-75 kDa

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  Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.