2. GPCR/G Protein
  3. GLP Receptor GCGR
  4. Retatrutide TFA

Retatrutide TFA  (Synonyms: LY3437943 TFA)

Cat. No.: HY-P3506A Purity: 99.05%
COA
SDS
Handling Instructions Technical Support

Retatrutide (LY3437943) TFA is a triple agonist peptide of the glucagon receptor (GCGR), glucosedependent insulinotropic polypeptide receptor (GIPR), and glucagon-like peptide-1 receptor (GLP-1R). Retatrutide TFA binds human GCGR, GIPR, and GLP-1R with EC50 values of 5.79, 0.0643 and 0.775 nM, respectively. Retatrutide TFA can be used for the research of obesity.

For research use only. We do not sell to patients.

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Retatrutide TFA:

Retatrutide TFA Related Antibodies

Top Publications Citing Use of Products
In Vivo Efficacy Study
Histological Imaging/Staining
IHC

    Retatrutide TFA purchased from MedChemExpress. Usage Cited in: NPJ Metab Health Dis. 2025;3(1):10.  [Abstract]

    Mice were subcutaneously administered either Veh, 30 nmol/kg Retatrutide, or 30 nmol/kg SEMA every other day before the initiation of the dark cycle at 5 pm. Survival curves were plotted as % of mice tumor-free for each group.

    Retatrutide TFA purchased from MedChemExpress. Usage Cited in: NPJ Metab Health Dis. 2025;3(1):10.  [Abstract]

    Mice were subcutaneously administered either Veh, 30 nmol/kg Retatrutide, or 30 nmol/kg SEMA every other day before the initiation of the dark cycle at 5 pm. Frequencies of CD45+ cells/live cells were shown.

    Retatrutide TFA purchased from MedChemExpress. Usage Cited in: Endocrine. 2025 Jan;87(1):159-169.  [Abstract]

    The mice received daily subcutaneous injections of 10 nmol/kg of Retatrutide for 10 weeks. Changes in fasting blood glucose (FBG) of mice in each group were recorded.

    Retatrutide TFA purchased from MedChemExpress. Usage Cited in: Endocrine. 2025 Jan;87(1):159-169.  [Abstract]

    The mice received daily subcutaneous injections of 10 nmol/kg of Retatrutide for 10 weeks. Representative HE and PAS stained images of the kidneys of mice in each group were obtained.

    Retatrutide TFA purchased from MedChemExpress. Usage Cited in: Endocrine. 2025 Jan;87(1):159-169.  [Abstract]

    The mice received daily subcutaneous injections of 10 nmol/kg of Retatrutide for 10 weeks. Representative immunohistochemical staining of TNF-α, Caspase-1, NLRP3, fibronectin, collagen I, and α-SMA in renal tissue of mice in each group was performed.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Retatrutide (LY3437943) TFA is a triple agonist peptide of the glucagon receptor (GCGR), glucosedependent insulinotropic polypeptide receptor (GIPR), and glucagon-like peptide-1 receptor (GLP-1R). Retatrutide TFA binds human GCGR, GIPR, and GLP-1R with EC50 values of 5.79, 0.0643 and 0.775 nM, respectively. Retatrutide TFA can be used for the research of obesity[1].

    IC50 & Target

    EC50 (for human): 5.79 (GCGR), 0.0643 (GIPR), 0.775 nM (GLP-1R)[1].
    EC50 (for mouse): 2.32 (GCGR), 0.191 (GIPR), 0.794 nM (GLP-1R) [1].
    Ki (for human): 5.6 (GCGR), 0.057 (GIPR), 7.2 nM (GLP-1)[1].
    In Vitro

    Retatrutide (LY3437943) TFA has efficacy for human GCGR, GIPR, and GLP-1R with EC50 values of 5.79, 0.0643 and 0.775 nM, respectively[1].
    Retatrutide has efficacy for mouse GCGR, GIPR, and GLP-1R with EC50 values of 2.32, 0.191 and 0.794 nM, respectively[1].
    Retatrutide has binding affinity for human GCGR, GIPR, and GLP-1R with Ki values of 5.6, 0.057 and 7.2 nM, respectively[1].
    Retatrutide has binding affinity for mouse GCGR, GIPR, and GLP-1R with Ki values of 73, 2.8 and 1.3 nM, respectively[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Retatrutide TFA Related Antibodies
    In Vivo

    Retatrutide (LY3437943) TFA (s.c.; 47 μg/kg; single) engages GCGR in vivo and can improve glucose tolerance in an ipGTT through either the GIP or GLP-1 receptors[1].
    Retatrutide (s.c.; 10 mL/kg; cycle every 3 days; for 21 days) causes great body weight loss and increases energy expenditure through glucagon receptor activatio[1].
    Retatrutide has safety and tolerability[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    4731.33 (free base)

    Formula

    C221H342N46O68.xC2HF3O2
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    Tyr-{Aib}-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Ile-{α-Me-Leu}-Leu-Asp-Lys-{diacid-C20-gamma-Glu-(AEEA)-Lys}-Ala-Gln-{Aib}-Ala-Phe-Ile-Glu-Tyr-Leu-Leu-Glu-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser-NH2
    Sequence Shortening

    Y-{Aib}-QGTFTSDYSI-{α-Me-Leu}-LDK-{Lys(AEEA-γGlu-C20 diacid)}AQ-{Aib}-AFIEYLLEGGPSSGAPPPS-NH2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL

    0.5 M NH4OH : 50 mg/mL (Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    Concentration (final)

    C2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 1.25 mg/mL; Clear solution

      This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.05%

    SDS (252 KB)

    COA (247 KB) LCMS (92 KB) Elemental Analysis Report (103 KB)

    Handling Instructions (2659 KB)
    References
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    Retatrutide TFA Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    RetatrutideLY3437943LY 3437943LY-3437943GLP ReceptorGCGRGlucagon Receptorpeptideglucagon receptor (GCGR)glucosedependent insulinotropic polypeptide receptor (GIPR)glucagon-like peptide-1 receptor (GLP-1R)obesityInhibitorinhibitorinhibit

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