2. GPCR/G Protein
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  4. Glucagon (1-29), bovine, human hydrochloride

Glucagon (1-29), bovine, human hydrochloride  (Synonyms: Glucagon hydrochloride)

Cat. No.: HY-P0082A Purity: 99.89%
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Glucagon (1-29), bovine, human, porcine hydrochloride is a peptide hormone, produced by pancreatic α-cells. Glucagon hydrochloride stimulates gluconeogenesis. Glucagon (1-29), bovine, human, porcine hydrochloride activates HNF4α and increases HNF4α phosphorylation.

For research use only. We do not sell to patients.

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CAS No. : 28270-04-4

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Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Glucagon (1-29), bovine, human hydrochloride:

Glucagon (1-29), bovine, human hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Glucagon (1-29), bovine, human hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Metab. 2025 Feb;7(2):367-382.  [Abstract]

    Glucose production in hepatocytes treated with proline, Glucagon (200 nM), or proline+glucagon for 24 h compared to control cells, n = 4.

    Glucagon (1-29), bovine, human hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Metab. 2025 Feb;7(2):367-382.  [Abstract]

    Neat1 level in hepatocytes treated with proline, Glucagon (200 nM), or proline+glucagon for 24 h, n = 4.

    Glucagon (1-29), bovine, human hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Metab. 2025 Feb;7(2):367-382.  [Abstract]

    Representative images showing cellular distribution of Neat1_2 (green) and NONO (red) in hepatocytes treated with proline, Glucagon (200 nM), or proline+glucagon for 24 h. The graph plots the fluorescence intensity of each probe or protein (y-axis) vs. distance (x-axis) for the corresponding white line.

    Glucagon (1-29), bovine, human hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Metab. 2025 Feb;7(2):367-382.  [Abstract]

    Quantification of paraspeckle numbers in hepatocytes treated with proline, Glucagon (200 nM), or proline+glucagon for 24 h, n = 40 cells per group.

    Glucagon (1-29), bovine, human hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Res. 2023 Apr;33(4):273-287.

    Effect of 10 min treatment with famsin (30 nM) or Glucagon (100 nM) on cellular cAMP levels in mouse primary hepatocytes.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Glucagon (1-29), bovine, human, porcine hydrochloride is a peptide hormone, produced by pancreatic α-cells. Glucagon hydrochloride stimulates gluconeogenesis[1]. Glucagon (1-29), bovine, human, porcine hydrochloride activates HNF4α and increases HNF4α phosphorylation[2][3].

    In Vitro

    Upon binding to its receptor Gcgr, Glucagon activates cAMP-PKA signaling to stimulate hepatic glucose production (HGP) and cause hyperglycemia[1].
    Glucagon stimulates both hepatic kisspeptin1 production and gluconeogenesis[1].
    Glucagon (100 nM) represses CYP7A1 mRNA expression in human primary hepatocytes[3].
    Glucagon (100 nM) increases phosphorylayion of HNF4α[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Glucagon (1-29), bovine, human hydrochloride Related Antibodies

    Western Blot Analysis[3]

    Cell Line: Human primary hepatocytes (H1211, HH1215)
    Concentration: 100 nM
    Incubation Time:
    Result: Resulted in a marked increase in the amount of phosphorylated HNF4α.
    In Vivo

    Low-dose (20 μg/kg) Glucagon increases glycemia and does not stimulate insulin secretion in ambient-fed mice. High-dose (1 mg/kg) Glucagon lowers glycemia compared with PBS control and stimulates insulin secretion in ambient-fed mice[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6J mice (12- to 24-week-old)[4]
    Dosage: 20 μg/kg and 1 mg/kg
    Administration: Administered by i.p. injection; 45 minutes
    Result: Low-dose (20 μg/kg) increased glycemia and did not stimulate insulin secretion.
    High-dose (1 mg/kg) lowered glycemia and stimulated insulin secretion.
    Clinical Trial
    Molecular Weight

    3519.21

    Formula

    C153H226ClN43O49S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr
    Sequence Shortening

    HSQGTFTSDYSKYLDSRRAQDFVQWLMNT
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (7.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 16.67 mg/mL (4.74 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.2842 mL 1.4208 mL 2.8415 mL
    5 mM 0.0568 mL 0.2842 mL 0.5683 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.89%

    SDS (252 KB)

    COA (242 KB) LCMS (100 KB) Elemental Analysis Report (102 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 0.2842 mL 1.4208 mL 2.8415 mL 7.1039 mL
    DMSO 5 mM 0.0568 mL 0.2842 mL 0.5683 mL 1.4208 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Glucagon (1-29), bovine, human hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Glucagon (1-29), bovine, human28270-04-4GlucagonGCGRGlucagon Receptorpeptidehormonepancreatic αgluconeogenesisHNF-4HNF4αInhibitorinhibitorinhibit

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