Glucagon (1-29), bovine, human (Synonyms: Glucagon)

Name: Glucagon (1-29), bovine, human
Brand: MedChemExpress
SKU: HY-P0082
Rating: 5.0 (11 reviews)

Cat. No.: HY-P0082 Purity: 99.91%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Glucagon (1-29), bovine, human, porcine is a peptide hormone, produced by pancreatic α-cells. Glucagon stimulates gluconeogenesis. Glucagon (1-29), bovine, human, porcine activates HNF4α and increases HNF4α phosphorylation.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 16941-32-5

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Glucagon (1-29), bovine, human:

Glucagon (1-29), bovine, human hydrochloride In-stock

11 Publications Citing Use of MCE Glucagon (1-29), bovine, human

Bio/Physico-chemical Assay

RT-PCR

Cell Imaging/Staining

: Glucagon (1-29), bovine, human purchased from MedChemExpress. Usage Cited in: Nat Metab. 2025 Feb;7(2):367-382. [Abstract]; Glucose production in hepatocytes treated with proline, Glucagon (200 nM), or proline+glucagon for 24 h compared to control cells, n = 4.

: Glucagon (1-29), bovine, human purchased from MedChemExpress. Usage Cited in: Nat Metab. 2025 Feb;7(2):367-382. [Abstract]; Neat1 level in hepatocytes treated with proline, Glucagon (200 nM), or proline+glucagon for 24 h, n = 4.

: Glucagon (1-29), bovine, human purchased from MedChemExpress. Usage Cited in: Nat Metab. 2025 Feb;7(2):367-382. [Abstract]; Representative images showing cellular distribution of Neat1_2 (green) and NONO (red) in hepatocytes treated with proline, Glucagon (200 nM), or proline+glucagon for 24 h. The graph plots the fluorescence intensity of each probe or protein (y-axis) vs. distance (x-axis) for the corresponding white line.

: Glucagon (1-29), bovine, human purchased from MedChemExpress. Usage Cited in: Nat Metab. 2025 Feb;7(2):367-382. [Abstract]; Quantification of paraspeckle numbers in hepatocytes treated with proline, Glucagon (200 nM), or proline+glucagon for 24 h, n = 40 cells per group.

: Glucagon (1-29), bovine, human purchased from MedChemExpress. Usage Cited in: Cell Res. 2023 Apr;33(4):273-287.; Effect of 10 min treatment with famsin (30 nM) or Glucagon (100 nM) on cellular cAMP levels in mouse primary hepatocytes.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Glucagon (1-29), bovine, human, porcine is a peptide hormone, produced by pancreatic α-cells. Glucagon stimulates gluconeogenesis^[1]. Glucagon (1-29), bovine, human, porcine activates HNF4α and increases HNF4α phosphorylation^[2]^[3].

In Vitro

Upon binding to its receptor Gcgr, Glucagon activates cAMP-PKA signaling to stimulate hepatic glucose production (HGP) and cause hyperglycemia^[1].
Glucagon stimulates both hepatic kisspeptin1 production and gluconeogenesis^[1].
Glucagon (100 nM) represses CYP7A1 mRNA expression in human primary hepatocytes^[3].
Glucagon (100 nM) increases phosphorylayion of HNF4α^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	Human primary hepatocytes (H1211, HH1215)
Concentration:	100 nM
Incubation Time:
Result:	Resulted in a marked increase in the amount of phosphorylated HNF4α.

In Vivo

Low-dose (20 μg/kg) Glucagon increases glycemia and does not stimulate insulin secretion in ambient-fed mice. High-dose (1 mg/kg) Glucagon lowers glycemia compared with PBS control and stimulates insulin secretion in ambient-fed mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6J mice (12- to 24-week-old)^[4]
Dosage:	20 μg/kg and 1 mg/kg
Administration:	Administered by i.p. injection; 45 minutes
Result:	Low-dose (20 μg/kg) increased glycemia and did not stimulate insulin secretion. High-dose (1 mg/kg) lowered glycemia and stimulated insulin secretion.

Molecular Weight

3482.75

Formula

C₁₅₃H₂₂₅N₄₃O₄₉S

CAS No.

16941-32-5

Appearance

Solid

Color

White to off-white

Sequence

His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr

Sequence Shortening

HSQGTFTSDYSKYLDSRRAQDFVQWLMNT

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 6.67 mg/mL (1.92 mM; ultrasonic and adjust pH to 3 with HCl)

DMSO : 2 mg/mL (0.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.2871 mL	1.4356 mL	2.8713 mL
5 mM	---	---	---
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.2 mg/mL (0.06 mM); Clear solution

This protocol yields a clear solution of ≥ 0.2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (2.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 0.2 mg/mL (0.06 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (2.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (286 KB) SDS (557 KB)

COA (259 KB) RP-HPLC (249 KB) MS (70 KB) Elemental Analysis Report (117 KB)

Handling Instructions (2659 KB)

References

[1]. Song WJ, et al. Glucagon regulates hepatic kisspeptin to impair insulin secretion. Cell Metab. 2014 Apr 1;19(4):667-81. [Content Brief]

[2]. Hirota K, et al. Hepatocyte nuclear factor-4 is a novel downstream target of insulin via FKHR as a signal-regulated transcriptional inhibitor. J Biol Chem. 2003 Apr 11;278(15):13056-60. [Content Brief]

[3]. Song KH, et al. Glucagon and cAMP inhibit cholesterol 7alpha-hydroxylase (CYP7A1) gene expression in humanhepatocytes: discordant regulation of bile acid synthesis and gluconeogenesis. Hepatology. 2006 Jan;43(1):117-25. [Content Brief]

[4]. Capozzi ME, et al. Glucagon lowers glycemia when β-cells are active. JCI Insight. 2019 Jul 23;5. pii: 129954. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O

1 mM

0.2871 mL

1.4356 mL

2.8713 mL

7.1782 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Glucagon (1-29), bovine, human Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Glucagon (1-29), bovine, human16941-32-5GlucagonGCGRGlucagon Receptorpeptidehormonepancreatic αgluconeogenesisHNF-4HNF4αInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Glucagon (1-29), bovine, human (Synonyms: Glucagon)

Customer Review

Other Forms of Glucagon (1-29), bovine, human:

Glucagon (1-29), bovine, human Related Antibodies

11 Publications Citing Use of MCE Glucagon (1-29), bovine, human

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Glucagon (1-29), bovine, human Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Glucagon (1-29), bovine, human Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report