Rhod-5N 

Rhod-5N is a cell-impermeant calcium-binding fluorescent dye composed of a BAPTA chelating group and a rhodamine fluorophore. Rhod-5N is often added to MOPS buffer to complex and indicate cation content. The selectivity of Rhod-5N for Cd²⁺ is higher than other interfering cations ((Na+, K+, Mg²⁺, Ca²⁺, Zn²⁺) except Pb²⁺), and the detection limit is 3.1 μg/L^[1][2].

Rhod-5N can be introduced into cells using methods such as patch clamping and microinjection.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

900.02

C₃₉H₃₆K₃N₅O₁₃

888229-31-0

CN(C1=CC2=C(C=C1)C(C(C=C3)=CC(OCCOC(C=C4[N+]([O-])=O)=C(C=C4)N(CC(O[K])=O)CC(O[K])=O)=C3N(CC(O[K])=O)CC([O-])=O)=C(C=CC(N(C)C)=C5)C5=[O+]2)C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Soibinet M, Souchon V, Leray I, et al. Rhod-5N as a fluorescent molecular sensor of cadmium (II) ion[J]. Journal of fluorescence, 2008, 18: 1077-1082.

  [2]. McNamara C J, Perry Iv T D, Bearce K, et al. Measurement of limestone biodeterioration using the Ca2+ binding fluorochrome Rhod-5N[J]. Journal of microbiological methods, 2005, 61(2): 245-250.