Rifamycin (Synonyms: Rifamycin SV)

Name: Rifamycin
Brand: MedChemExpress
SKU: HY-B1907A
Rating: 5.0 (2 reviews)

Cat. No.: HY-B1907A Purity: 98.06%: Data Sheet
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Rifamycin (Rifamycin SV) is an orally active ansamycin antibiotic. Rifamycin inhibits DNA-dependent RNA synthesis. Rifamycin has antibacterial activity against Mycobacterium tuberculosis. Rifamycin interferes with hepatic bile acid metabolism. Rifamycin has anti-inflammatory effects. Rifamycin can be used in the study of Mycobacterium tuberculosis, Bacteroides fragilis infection, and Lipopolysaccharide (HY-D1056B3)-induced inflammation.

For research use only. We do not sell to patients.

Rifamycin Chemical Structure

CAS No. : 6998-60-3

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Rifamycin:

Rifamycin S In-stock
Rifamycin sodium In-stock

2 Publications Citing Use of MCE Rifamycin

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Cancer cell lines	GI₅₀	10 μM Compound: Rifamycin SV	Growth inhibition of human Colon cancer cells Growth inhibition of human Colon cancer cells	[PMID: 25205189]
Cancer cell lines	GI₅₀	10 μM Compound: Rifamycin SV	Growth inhibition of human Prostate cancer cells Growth inhibition of human Prostate cancer cells	[PMID: 25205189]
Cancer cell lines	GI₅₀	15.9 μM Compound: Rifamycin SV	Growth inhibition of human Renal cancer cells Growth inhibition of human Renal cancer cells	[PMID: 25205189]
Cancer cell lines	GI₅₀	16.6 μM Compound: Rifamycin SV	Growth inhibition of human Breast cancer cells Growth inhibition of human Breast cancer cells	[PMID: 25205189]
Cancer cell lines	GI₅₀	18.6 μM Compound: Rifamycin SV	Growth inhibition of human Ovarian cancer cells Growth inhibition of human Ovarian cancer cells	[PMID: 25205189]
Cancer cell lines	GI₅₀	21.4 μM Compound: Rifamycin SV	Growth inhibition of human CNS cancer cells Growth inhibition of human CNS cancer cells	[PMID: 25205189]
HEK293	IC₅₀	0.23 μM Compound: Rifamycin SVd	TP_TRANSPORTER: inhibition of estradiol-17beta-glucuronide uptake(estradiol-17beta-glucuronide:0.02uM) in OATP1B1-expressing HEK293 cells TP_TRANSPORTER: inhibition of estradiol-17beta-glucuronide uptake(estradiol-17beta-glucuronide:0.02uM) in OATP1B1-expressing HEK293 cells	[PMID: 15616150]
Leukemia cell	GI₅₀	3.2 μM Compound: Rifamycin SV	Growth inhibition of human Leukemia cells Growth inhibition of human Leukemia cells	[PMID: 25205189]
Melanoma cell	GI₅₀	15.5 μM Compound: Rifamycin SV	Growth inhibition of human Melanoma cells Growth inhibition of human Melanoma cells	[PMID: 25205189]
NSCLC	GI₅₀	14.5 μM Compound: Rifamycin SV	Growth inhibition of human NSCLC cells Growth inhibition of human NSCLC cells	[PMID: 25205189]
Sf21	IC₅₀	3.1 μM Compound: Rifamycin SV	Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]
Sf21	IC₅₀	6.3 μM Compound: Rifamycin SV	Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]
Vero	CC₅₀	62.46 μg/mL Compound: RFM	Cytotoxicity against african green monkey Vero cells after 72 hrs by resazurin assay Cytotoxicity against african green monkey Vero cells after 72 hrs by resazurin assay	[PMID: 23474387]

In Vitro

Rifamycin (10 μM; 30 s) mainly inhibits -independent Taurocholate (HY-N0545) uptake in short-term cultured rat hepatocytes^[2].
Rifamycin (10-100 μM; 15 min) effectively inhibits Oatp2-mediated Taurocholate uptake in Xenopus oocytes expressing Na⁺/Ntcp^[2].
Rifamycin (1-100 μM; 24 h) inhibits the synthesis of cytokines and chemokines from lipopolysaccharide-activated monocytes and macrophages^[3].
Rifamycin (0.16 μg/ml; 2 weeks of culture) inhibits the growth of M. tuberculosis and its drug-resistant mutants in TB broth containing 10% serum albumin^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Rifamycin (5 mg/day; s.c.; 3 days a week) is effective in mice infected with M. tuberculosis, significantly reducing the number of viable bacteria in the body^[4].
Rifamycin (12.5-25 mg/kg; peritoneal lavage) can improve the survival rate of rats with experimental intraperitoneal infection and significantly reduce the number of intraperitoneal bacteria and adhesion formation^[6].
Rifamycin (5-40 mg/kg; esophageal gavage; once a day, 5 days a week; 4 weeks) shortens oral treatment duration in a mouse model of Mycobacterium ulcerans disease^[8].
Rifamycin (0.1 mL; intraaural administration; twice daily; 10 days) does not cause hearing loss in adult or weanling rats^[9].
Rifamycin (1 mg i.v. bolus followed by 4 mg i.v. infusion; 70 min) interferes with three major steps of Bile acid metabolism in rats with intravenous Sodium cholate (HY-N0324A) infusion, resulting in a significant decrease in bile acid uptake and excretion^[10].
Rifamycin (10-160 mg/kg; s.c.; single dose) is approximately 11 times less effective than Metronidazole (HY-B0318) in a mouse Bacteroides fragilis thigh infection model^[11].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats (weight 200-250 g), cecal ligation puncture (CLP)-induced intra-abdominal infection model^[6]
Dosage:	25 mg/kg, 12.5 mg/kg
Administration:	Peritoneal lavage
Result:	Improved survival from 50% in the control group to 91.7% in the 25 mg/kg group and 100% in the 12.5 mg/kg group. Significantly reduced adhesion formation. Showed a greater reduction in bacterial counts in peritoneal fluid (25 mg/kg).

Molecular Weight

697.77

Formula

C₃₇H₄₇NO₁₂

CAS No.

6998-60-3

Appearance

Solid

Color

Light yellow to brown

SMILES

C[C@H]1/C=C/C=C(C)\C(NC2=CC(O)=C3C(C(O)=C(C)C4=C3C([C@](O/C=C/[C@H](OC)[C@@H](C)[C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@H]1O)(C)O4)=O)=C2O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (143.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4331 mL	7.1657 mL	14.3314 mL
5 mM	0.2866 mL	1.4331 mL	2.8663 mL
10 mM	0.1433 mL	0.7166 mL	1.4331 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (7.17 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (7.17 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.06%

Select Batch:

Data Sheet (289 KB) SDS (393 KB)

COA (260 KB) HNMR (565 KB) RP-HPLC (261 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Floss HG, et al. Rifamycin-mode of action, resistance, and biosynthesis. Chem Rev. 2005 Feb;105(2):621-32. [Content Brief]

[2]. Fattinger K, et al. Rifamycin SV and rifampicin exhibit differential inhibition of the hepatic rat organic anion transporting polypeptides, Oatp1 and Oatp2. Hepatology. 2000 Jul;32(1):82-6. [Content Brief]

[3]. Rosette C, et al. Anti-inflammatory and immunomodulatory activities of rifamycin SV. Int J Antimicrob Agents. 2013 Aug;42(2):182-6. [Content Brief]

[4]. Tsukamura M, et al. Antituberculous Action of Rifamycin SV* In Vitro and In Vivo Studies. The Journal of Antibiotics, Series A, 1962, 15(5): 216-224.

[5]. Saito K, et al. Rifamycin action on RNA polymerase in antibiotic-tolerant Mycobacterium tuberculosis results in differentially detectable populations. Proc Natl Acad Sci U S A. 2017 Jun 13;114(24):E4832-E4840. [Content Brief]

[6]. Jallouli M, et al. Rifamycin lavage in the treatment of experimental intra-abdominal infection. J Surg Res. 2009 Aug;155(2):191-4. [Content Brief]

[7]. Zhang M, et al. Treatment of tuberculosis with rifamycin-containing regimens in immune-deficient mice. Am J Respir Crit Care Med. 2011 May 1;183(9):1254-61. [Content Brief]

[8]. Omansen TF, et al. High-Dose Rifamycins Enable Shorter Oral Treatment in a Murine Model of Mycobacterium ulcerans Disease. Antimicrob Agents Chemother. 2019 Jan 29;63(2):e01478-18. [Content Brief]

[9]. Özbay C, et al. Ototoxic effect of topical rifamycin SV applied in the middle ear of rats. The Turkish Journal of Ear Nose and Throat, 2017, 27(1): 31-38.

[10]. Okolicsanyi L, et al. Influence of rifamycin SV on bile acid metabolism in rats. Naunyn Schmiedebergs Arch Pharmacol. 1980 Aug;313(2):171-4. [Content Brief]

[11]. Dijkmans B A C, et al. Efficacy of rifamycin SV and vancomycin against Bacteroides fragilis in vitro and in experimentally infected mice. Current Microbiology, 1985, 12: 53-58.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4331 mL	7.1657 mL	14.3314 mL	35.8284 mL
	5 mM	0.2866 mL	1.4331 mL	2.8663 mL	7.1657 mL
	10 mM	0.1433 mL	0.7166 mL	1.4331 mL	3.5828 mL
	15 mM	0.0955 mL	0.4777 mL	0.9554 mL	2.3886 mL
	20 mM	0.0717 mL	0.3583 mL	0.7166 mL	1.7914 mL
	25 mM	0.0573 mL	0.2866 mL	0.5733 mL	1.4331 mL
	30 mM	0.0478 mL	0.2389 mL	0.4777 mL	1.1943 mL
	40 mM	0.0358 mL	0.1791 mL	0.3583 mL	0.8957 mL
	50 mM	0.0287 mL	0.1433 mL	0.2866 mL	0.7166 mL
	60 mM	0.0239 mL	0.1194 mL	0.2389 mL	0.5971 mL
	80 mM	0.0179 mL	0.0896 mL	0.1791 mL	0.4479 mL
	100 mM	0.0143 mL	0.0717 mL	0.1433 mL	0.3583 mL

Rifamycin Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rifamycin6998-60-3Rifamycin SVAntibioticBacterialDNA/RNA SynthesisM. tuberculosis ratsmonocytes and macrophagesanti-inflammatoryantibacterialInhibitorinhibitorinhibit

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Rifamycin (Synonyms: Rifamycin SV)

Customer Review

Other Forms of Rifamycin:

Rifamycin Related Antibodies

2 Publications Citing Use of MCE Rifamycin

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Biological Activity

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References

Customer Review

Rifamycin Related Antibodies

Molarity Calculator

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Complete Stock Solution Preparation Table

Rifamycin Related Classifications

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