Scopolamine hydrochloride
Based on 2 publication(s) in Google Scholar
Scopolamine (Hyoscine) hydrochloride is a high-affinity muscarinic antagonist that can cross the blood-brain barrier. Scopolamine hydrochloride competitively antagonizes 5-HT3 receptors with an IC50 of 2.09 μM. Scopolamine hydrochloride can induce cognitive and memory deficits in animals. Scopolamine hydrochloride can be used in the research of preventing postoperative nausea and vomiting, motion sickness, nervous system diseases, etc.
For research use only. We do not sell to patients.
- Purity: 99.66%
- CAS No.: 55-16-3
- Formula: C17H22ClNO4
- Molecular Weight:339.81
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Scopolamine hydrochloride
MoreAll 5-HT Receptor Isoforms
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Biological Activity
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5-HT3 Receptor 2.09 μM (IC50) |
Scopolamine hydrochloride blocks nicotinic acetylcholine receptors (IC50 = 928 μM) and increases the expression of α7 nACh receptors. Scopolamine hydrochloride displays concentration-dependent competition with 0.6 nM [3H]Granisetron, yielding an average pKi of 5.17 (Ki = 6.76 μM). Concentration-dependent competition of fluorescently labeled form of Granisetron (HY-B0071) with Scopolamine hydrochloride gives an average pKi of 5.31 (Ki = 4.90 μM).Scopolamine hydrochloride blocks muscarinic receptors and induces a cognitive deficit[1].
Scopolamine (0.1-3 mM; for 24 hours) hydrochloride induces cytotoxicity, ROS generation and apoptosis of SH-SY5Y cells[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human neuroblastoma SH-SY5Y cell line
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Concentration:0.1-3 mM (usually 2 mM)
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Incubation Time:24 hours
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Result:Exposure to 1-3 mM for 24 hours significantly decreased human neuroblastoma SH-SY5Y cell viability in a dose-dependent manner. In all subsequent experiments, 2 mM resulted in 50.6% loss in cell viability.
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Animal Model:Naive male Swiss mice weighing 20-25[7]
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Dosage:1 mg/kg
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Administration:Administered intraperitoneally (i.p.); to measure the memory acquisition processes, Scopolamine was administered 20 min before the pretest; to measure the memory consolidation processes, Scopolamine was administered immediately after the pretest
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Result:There was a significant decrease in the index of latency (IL) value as compared with the saline-treated mice, at the used dose impaired acquisition of memory and learning.
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.
Scopolamine (1 mg/kg; i.p.; once) induces memory impairment associated with attenuation of cholinergic neurotransmission, as well as an increases of processes connected with oxidative stress in the brain in mice[7].
Scopolamine can be used to induce memory impairment models[4].
Administration: 1 mg/kg/day for 7 days
Behavior-Locomotion activity in open field: Decreased time spent in the centre of the open field. Decreased number of crossing and rearing.
Behavior-Object recognition: Increased time to discover and stay with the familiar object. Decreased number of exploration of the novel object.
Molecular changes: Decreased level of glutathione. Increased activity of acetylcholinesterase in brain.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 55-16-3
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Appearance Solid
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Molecular Weight 339.81
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Formula C17H22ClNO4
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Color White to off-white
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SMILES
O=C([C@@H](C1=CC=CC=C1)CO)O[C@H]2C[C@H]3[C@@H]4O[C@@H]4[C@H](N3C)C2.[H]Cl
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Synonyms
(-)-Scopolamine hydrochloride; Hyoscine hydrochloride
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Eur J Pharmacol
Targeting microglial GLP1R in epilepsy: A novel approach to modulate neuroinflammation and neuronal apoptosis. [Abstract]2024 Aug 16:176903. PMID: 39154823 -
J Dig Dis
DREADDs-Based Chemogenetics Induced Slow Transit Constipation via Inhibition of Enteric Neurons. [Abstract]2025 Apr 14. PMID: 40223443
Solvent & Solubility
H2O : 50 mg/mL (147.14 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (285 KB)
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SDS (642 KB)
- English - EN (642 KB)
- Français - FR (642 KB)
- Deutsch - DE (642 KB)
- Norwegian - NO (642 KB)
- Español - ES (642 KB)
- Swedish - SV (642 KB)
- Italian - IT (642 KB)
- Korean - KR (642 KB)
- Portuguese - PT (642 KB)
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Handling Instructions (2659 KB)
References
[1]. Lochner M, et al. The muscarinic antagonists Scopolamine and atropine are competitive antagonists at 5-HT3 receptors. Neuropharmacology. 2016 Sep;108:220-8. [Content Brief]
[2]. O ET, et al. COGNITIVE-ENHANCING PROPERTIES OF MORINDA LUCIDA (RUBIACEAE) AND PELTOPHORUM PTEROCARPUM (FABACEAE) IN SCOPOLAMINE-INDUCED AMNESIC MICE. Afr J Tradit Complement Altern Med. 2017 Mar 1;14(3):136-141. [Content Brief]
[3]. Pattanashetti LA, et al. Evaluation of neuroprotective effect of Quercetin with Donepezil in Scopolamine-induced amnesia in rats. Indian J Pharmacol. 2017 Jan-Feb;49(1):60-64. [Content Brief]
[4]. Yadang FSA, et al. Scopolamine-Induced Memory Impairment in Mice: Neuroprotective Effects of Carissa edulis (Forssk.) Valh (Apocynaceae) Aqueous Extract. Int J Alzheimers Dis. 2020 Aug 31;2020:6372059. [Content Brief]
[5]. Klinkenberg I, et al. The validity of scopolamine as a pharmacological model for cognitive impairment: a review of animal behavioral studies. Neurosci Biobehav Rev. 2010 Jul;34(8):1307-50. [Content Brief]
[6]. Nicha Puangmalai, et al. Neuroprotection of N-benzylcinnamide on scopolamine-induced cholinergic dysfunction in human SH-SY5Y neuroblastoma cells. Neural Regen Res. 2017 Sep;12(9):1492-1498. [Content Brief]
[7]. Barbara Budzynska, et al. Effects of imperatorin on scopolamine-induced cognitive impairment and oxidative stress in mice. Psychopharmacology (Berl). 2015 Mar;232(5):931-42. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 2.9428 mL | 14.7141 mL | 29.4282 mL | 73.5705 mL |
| 5 mM | 0.5886 mL | 2.9428 mL | 5.8856 mL | 14.7141 mL | |
| 10 mM | 0.2943 mL | 1.4714 mL | 2.9428 mL | 7.3571 mL | |
| 15 mM | 0.1962 mL | 0.9809 mL | 1.9619 mL | 4.9047 mL | |
| 20 mM | 0.1471 mL | 0.7357 mL | 1.4714 mL | 3.6785 mL | |
| 25 mM | 0.1177 mL | 0.5886 mL | 1.1771 mL | 2.9428 mL | |
| 30 mM | 0.0981 mL | 0.4905 mL | 0.9809 mL | 2.4524 mL | |
| 40 mM | 0.0736 mL | 0.3679 mL | 0.7357 mL | 1.8393 mL | |
| 50 mM | 0.0589 mL | 0.2943 mL | 0.5886 mL | 1.4714 mL | |
| 60 mM | 0.0490 mL | 0.2452 mL | 0.4905 mL | 1.2262 mL | |
| 80 mM | 0.0368 mL | 0.1839 mL | 0.3679 mL | 0.9196 mL | |
| 100 mM | 0.0294 mL | 0.1471 mL | 0.2943 mL | 0.7357 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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