Sodium phosphate dibasic, for molecular biology
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Sodium phosphate dibasic (Disodium hydrogen phosphate), for molecular biology, is a type of phosphate salt that can be used in molecular biology research to prepare stable buffers free of RNase/DNase/protease.
For research use only. We do not sell to patients.
- Purity: 99.65%
- CAS No.: 7558-79-4
- Formula: HNa2O4P
- Molecular Weight:141.96
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Storage:
RT, sealed storage, away from moisture and light.
In solvent -80°C, 1 year , -20°C, 6 months
Biological Activity
Description
In Vitro
Sodium phosphate dibasic, for molecular biology is a key component of phosphate buffer solution (PBS), cell culture medium, protein purification and nucleic acid extraction, and it can maintain the stability of pH value[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 7558-79-4
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Appearance Solid
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Molecular Weight 141.96
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Formula HNa2O4P
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Color White to off-white
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SMILES
[Na2HPO4]
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Synonyms
Disodium hydrogen phosphate, for molecular biology
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, sealed storage, away from moisture and light
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
In Vitro:
H2O : 100 mg/mL (704.42 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (268 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 7.0442 mL | 35.2212 mL | 70.4424 mL | 176.1059 mL |
| 5 mM | 1.4088 mL | 7.0442 mL | 14.0885 mL | 35.2212 mL | |
| 10 mM | 0.7044 mL | 3.5221 mL | 7.0442 mL | 17.6106 mL | |
| 15 mM | 0.4696 mL | 2.3481 mL | 4.6962 mL | 11.7404 mL | |
| 20 mM | 0.3522 mL | 1.7611 mL | 3.5221 mL | 8.8053 mL | |
| 25 mM | 0.2818 mL | 1.4088 mL | 2.8177 mL | 7.0442 mL | |
| 30 mM | 0.2348 mL | 1.1740 mL | 2.3481 mL | 5.8702 mL | |
| 40 mM | 0.1761 mL | 0.8805 mL | 1.7611 mL | 4.4026 mL | |
| 50 mM | 0.1409 mL | 0.7044 mL | 1.4088 mL | 3.5221 mL | |
| 60 mM | 0.1174 mL | 0.5870 mL | 1.1740 mL | 2.9351 mL | |
| 80 mM | 0.0881 mL | 0.4403 mL | 0.8805 mL | 2.2013 mL | |
| 100 mM | 0.0704 mL | 0.3522 mL | 0.7044 mL | 1.7611 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.