Potassium dihydrogen phosphate, for cell culture
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Potassium dihydrogen phosphate, for cell culture (Potassium phosphate monobasic, for cell culture) is a cell culture reagent. Potassium dihydrogen phosphate, for cell culture activates NF-κB. Potassium dihydrogen phosphate, for cell culture upregulates the expression of dental/osteogenic markers (OCN, DSP/DSPP, OSX, RUNX2, ALP) and enhances the mineralization capacity of human periodontal ligament stem cells. Potassium dihydrogen phosphate, for cell culture promotes the proliferation of human periodontal ligament stem cells in logarithmic growth phase. Potassium dihydrogen phosphate, for cell culture promotes the growth of somatic embryos of Dendrobium Sonia, increases leaf number, leaf length and fresh weight of tissue-cultured seedlings. Potassium dihydrogen phosphate, for cell culture is applicable to periodontal disease-related research.
For research use only. We do not sell to patients.
- CAS No.: 7778-77-0
- Formula: H2KO4P
- Molecular Weight:136.09
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
Biological Activity
Description
IC50 & Target
[1]|
RUNX2 |
In Vitro
Potassium dihydrogen phosphate (1.8 mmol/L; treated on days 0, 1, 3, 5, 7, and 9) promotes the proliferation of PDLSCs in the logarithmic growth phase[1].
Potassium dihydrogen phosphate (1.8 mmol/L; 3 or 7 days) exerts no significant effect on the apoptosis rate of PDLSCs[1].
Potassium dihydrogen phosphate (1.8 mmol/L; 3 or 7 days) upregulates dentin/osteogenesis-related proteins in PDLSCs[1].
Potassium dihydrogen phosphate (180 mg/L) significantly promotes the growth of somatic embryo explants of *Dendrobium* 'Sonia' in MS medium. After 3 months of culture, each explant produces an average of 4.41 leaves with a leaf length of 1.16 cm, and the fresh weight of the tissue-cultured seedlings reaches 0.10 g[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human periodontal ligament stem cells (PDLSCs)
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Concentration:1.8 mmol/L
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Incubation Time:3 d, 7 d
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Result:Increased the expression of odonto/osteogenic proteins (OCN, DSP, OSX, RUNX2, ALP) at day 3 and 7 compared to control (P < 0.05, P < 0.01).
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Cell Line:human periodontal ligament stem cells (PDLSCs)
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Concentration:1.8 mmol/L
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Incubation Time:3 d, 7 d
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Result:Increased the expression of odonto/osteogenic genes (OCN, DSP, OSX, RUNX2, ALP) at day 3 and 7 compared to control (P < 0.05, P < 0.01).
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Cell Line:human periodontal ligament stem cells (PDLSCs)
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Concentration:1.8 mmol/L
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Incubation Time:15 min, 30 min, 60 min, 120 min
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Result:Down-regulated IκBα expression, reaching minimum at 30 min (P < 0.01).
Up-regulated nuclear P65 expression at 60 min (P < 0.01).
Chemical Information
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CAS No. 7778-77-0
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Appearance Solid
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Molecular Weight 136.09
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Formula H2KO4P
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Color White to off-white
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SMILES
O=P(O[O])=O.[KH2]
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Synonyms
Potassium phosphate monobasic, for cell culture
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
In Vitro:
H2O : 50 mg/mL (367.40 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 7.3481 mL | 36.7404 mL | 73.4808 mL | 183.7020 mL |
| 5 mM | 1.4696 mL | 7.3481 mL | 14.6962 mL | 36.7404 mL | |
| 10 mM | 0.7348 mL | 3.6740 mL | 7.3481 mL | 18.3702 mL | |
| 15 mM | 0.4899 mL | 2.4494 mL | 4.8987 mL | 12.2468 mL | |
| 20 mM | 0.3674 mL | 1.8370 mL | 3.6740 mL | 9.1851 mL | |
| 25 mM | 0.2939 mL | 1.4696 mL | 2.9392 mL | 7.3481 mL | |
| 30 mM | 0.2449 mL | 1.2247 mL | 2.4494 mL | 6.1234 mL | |
| 40 mM | 0.1837 mL | 0.9185 mL | 1.8370 mL | 4.5925 mL | |
| 50 mM | 0.1470 mL | 0.7348 mL | 1.4696 mL | 3.6740 mL | |
| 60 mM | 0.1225 mL | 0.6123 mL | 1.2247 mL | 3.0617 mL | |
| 80 mM | 0.0919 mL | 0.4593 mL | 0.9185 mL | 2.2963 mL | |
| 100 mM | 0.0735 mL | 0.3674 mL | 0.7348 mL | 1.8370 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.