Magnesium sulfate heptahydrate, for cell culture
Based on 1 Customer Validation
Magnesium sulfate heptahydrate, for cell culture (Epsom salts, for cell culture) is a hydrate of magnesium sulfate (HY-Y1267) suitable for cell culture applications. Magnesium sulfate is a calcium antagonist and a potent L-type calcium channel inhibitor, as well as a tocolytic. Magnesium sulfate has anti-inflammatory, anticonvulsant, vasodilatory, and neuroprotective effects. Magnesium sulfate can be used in the research of diseases such as preeclampsia/eclampsia.
For research use only. We do not sell to patients.
- Purity : 99.0%
- CAS No.: 10034-99-8
- Formula: MgSO4.7H2O
- Molecular Weight:246.47
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-Y0344D
Sodium chloride, for cell culture -
HY-108536D
Calcium chloride dihydrate, for cell culture, 99% -
HY-Y0537E
Potassium chloride, for cell culture -
HY-Y0537
Potassium chloride, AR, 99.5% -
HY-B0389
D-Glucose -
HY-W019883
Dipotassium hydrogen phosphate -
HY-Y0332K
Potassium dihydrogen phosphate, for cell culture -
HY-111391
Resazurin sodium -
HY-Y1891
Tween 80 -
HY-B2243
Anhydrous sodium dihydrogen phosphate -
HY-Y1369C
Manganese sulfate monohydrate, for cell culture -
HY-W019981B
Magnesium chloride hexahydrate, for molecular biology
Biological Activity
Description
In Vitro
Magnesium sulfate (3 mM) inhibits phosphatidylinositol-4,5-bisphosphate-specific phospholipase C activity and subsequent calcium release in cultured myometrial cells[2].
Magnesium sulfate (20 mM; 6 h) inhibits the LPS (HY-D1056)-induced inflammatory molecules production and NF-κB activation in RAW264.7 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW264.7 cells treated with 100 ng/mL of LPS (HY-D1056)
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Concentration:20 mM
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Incubation Time:6 h
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Result:Inhibtied the levels of iNOS and COX-2.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CD-1 out-bred timed pregnant mice, and intrauterine inflammation was induced by intrauterine injection of LPS (HY-D1056) on embryonic day 15[4]
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Dosage:270 mg/kg, followed by 27 mg/kg every 20 minutes for 4 hours, and then an additional 270 mg/kg
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Administration:Intraperitoneal injection
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Result:Prevented LPS-induced neuronal injury in fetal mouse brains, as evidenced by maintaining normal neuronal morphology, including dendritic processes and MAP2 staining, without significantly affecting the expression of proinflammatory cytokines or cell death markers.
Chemical Information
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CAS No. 10034-99-8
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Appearance Solid
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Molecular Weight 246.47
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Formula MgSO4.7H2O
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Color White to off-white
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SMILES
[MgSO4 ?7H2O]
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Synonyms
Epsom salts, for cell culture
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
In Vitro:
H2O : 200 mg/mL (811.46 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Ramsey PS, et al. Magnesium sulfate as a tocolytic agent. Semin Perinatol. 2001;25(4):236-247. [Content Brief]
[2]. Hurd WW, et al. Magnesium sulfate inhibits the oxytocin-induced production of inositol 1,4,5-trisphosphate in cultured human myometrial cells. Am J Obstet Gynecol. 2002 Aug;187(2):419-24. [Content Brief]
[3]. Lin CY, et al. L-type calcium channels are involved in mediating the anti-inflammatory effects of magnesium sulphate. Br J Anaesth. 2010 Jan;104(1):44-51. [Content Brief]
[4]. Burd I, et al. Magnesium sulfate reduces inflammation-associated brain injury in fetal mice. Am J Obstet Gynecol. 2010 Mar;202(3):292.e1-9. [Content Brief]
[5]. Okusanya BO, et al. Clinical pharmacokinetic properties of magnesium sulphate in women with pre-eclampsia and eclampsia. BJOG. 2016;123(3):356-366. [Content Brief]
[6]. Hunter LA, et al. Magnesium sulfate: past, present, and future. J Midwifery Womens Health. 2011 Nov-Dec;56(6):566-74. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.0573 mL | 20.2864 mL | 40.5729 mL | 101.4322 mL |
| 5 mM | 0.8115 mL | 4.0573 mL | 8.1146 mL | 20.2864 mL | |
| 10 mM | 0.4057 mL | 2.0286 mL | 4.0573 mL | 10.1432 mL | |
| 15 mM | 0.2705 mL | 1.3524 mL | 2.7049 mL | 6.7621 mL | |
| 20 mM | 0.2029 mL | 1.0143 mL | 2.0286 mL | 5.0716 mL | |
| 25 mM | 0.1623 mL | 0.8115 mL | 1.6229 mL | 4.0573 mL | |
| 30 mM | 0.1352 mL | 0.6762 mL | 1.3524 mL | 3.3811 mL | |
| 40 mM | 0.1014 mL | 0.5072 mL | 1.0143 mL | 2.5358 mL | |
| 50 mM | 0.0811 mL | 0.4057 mL | 0.8115 mL | 2.0286 mL | |
| 60 mM | 0.0676 mL | 0.3381 mL | 0.6762 mL | 1.6905 mL | |
| 80 mM | 0.0507 mL | 0.2536 mL | 0.5072 mL | 1.2679 mL | |
| 100 mM | 0.0406 mL | 0.2029 mL | 0.4057 mL | 1.0143 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Magnesium sulfate heptahydrate, for cell culture Related Classifications
HY-W094475C Related Classifications
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