Calcium chloride dihydrate, for cell culture, 99%
Based on 1 Customer Validation
Calcium chloride dihydrate, for cell culture, 99% is a biochemical reagent. Calcium chloride dihydrate, for cell culture, 99% enhances the dentinogenic differentiation potential of WMTA on cells. Calcium chloride dihydrate, for cell culture, 99% can be used as a biological material or organic compound for life science related research[2.
For research use only. We do not sell to patients.
- Purity : 99.86%
- CAS No.: 10035-04-8
- Formula: CaCl2H4O2
- Molecular Weight:147.01
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Biological Activity
Description
In Vitro
Calcium chloride dihydrate, for cell culture, 99% (12.5-200 mg/mL, 24-72 h) increases the cytotoxicity but enhances the dentinogenic differentiation potential of WMTA on dental pulp stem cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Dental pulp stem cells (DPSCs)
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Concentration:200, 100, 50, 25, and 12.5 mg/mL, FS WMTA (extracts of WMTA/calcium chloride dihydrate combination)
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Incubation Time:24 h, 72 h
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Result:Showed severe cytotoxic activity at two successive concentrations (200 and 100 mg/ml) after 24 and 72 h.
Showed moderate cytotoxic activity at 50 mg/mL.
Showed noncytotoxic activities at 12.5 mg/mL.
Chemical Information
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CAS No. 10035-04-8
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Appearance Solid
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Molecular Weight 147.01
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Formula CaCl2H4O2
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Color White to off-white
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SMILES
Cl[Ca]Cl.O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
H2O : ≥ 100 mg/mL (680.23 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 6.8023 mL | 34.0113 mL | 68.0226 mL | 170.0565 mL |
| 5 mM | 1.3605 mL | 6.8023 mL | 13.6045 mL | 34.0113 mL | |
| 10 mM | 0.6802 mL | 3.4011 mL | 6.8023 mL | 17.0056 mL | |
| 15 mM | 0.4535 mL | 2.2674 mL | 4.5348 mL | 11.3371 mL | |
| 20 mM | 0.3401 mL | 1.7006 mL | 3.4011 mL | 8.5028 mL | |
| 25 mM | 0.2721 mL | 1.3605 mL | 2.7209 mL | 6.8023 mL | |
| 30 mM | 0.2267 mL | 1.1337 mL | 2.2674 mL | 5.6685 mL | |
| 40 mM | 0.1701 mL | 0.8503 mL | 1.7006 mL | 4.2514 mL | |
| 50 mM | 0.1360 mL | 0.6802 mL | 1.3605 mL | 3.4011 mL | |
| 60 mM | 0.1134 mL | 0.5669 mL | 1.1337 mL | 2.8343 mL | |
| 80 mM | 0.0850 mL | 0.4251 mL | 0.8503 mL | 2.1257 mL | |
| 100 mM | 0.0680 mL | 0.3401 mL | 0.6802 mL | 1.7006 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.