Ammonium sulphate,≥99.0%,AR
Based on 1 Customer Validation
Ammonium sulphate,≥99.0%,AR is an inorganic sulfate salt used for molecular biology. Ammonium sulphate,≥99.0%,AR can be used to precipitate proteins, separate antibodies, and enhance antigen-antibody reactions. Ammonium sulphate,≥99.0%,AR can serve as a nitrogen source in plant fertilizers and has developmentally toxicity to freshwater snails, but increases the number of Anopheles africanus and Culex mosquito larvae in rice paddies. Ammonium sulphate,≥99.0%,AR also inhibits the conversion of glucose to ascorbic acid in plants.
For research use only. We do not sell to patients.
- Purity: 99.3%
- CAS No.: 7783-20-2
- Formula: (NH4)2SO4
- Molecular Weight:132.14
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
Biological Activity
Ammonium sulphate,≥99.0%,AR shows LC50 of 558, 393 and 701 mg/L for eggs, juveniles and adults of Helisoma trivolvis and 669, 526 and 657 mg/L for Biomphalaria havanensis[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 7783-20-2
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Appearance Solid
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Molecular Weight 132.14
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Formula (NH4)2SO4
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Color White to off-white
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SMILES
[(NH4)2SO4]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
H2O : 100 mg/mL (756.77 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Wingfield P,et al. Protein precipitation using ammonium sulfate. Curr Protoc Protein Sci. 2001 May;Appendix 3:Appendix 3F. [Content Brief]
[2]. Fishman JB,et al. Ammonium Sulfate Fractionation of Antibodies. Cold Spring Harb Protoc. 2018 Jun 1;2018(6). [Content Brief]
[3]. Stakenburg J,et al. Ammonium sulphate enhancement of the antigen-antibody reaction. Manual and automated nephelometric studies. Clin Chim Acta. 1979 Feb 1;91(3):251-61. [Content Brief]
[4]. Tchounwou PB, et al. Toxicity evaluation of ammonium sulphate and urea to three developmental stages of freshwater snails. Arch Environ Contam Toxicol. 1991 Sep;21(3):359-64. [Content Brief]
[5]. Mutero CM, et al. Ammonium sulphate fertiliser increases larval populations of Anopheles arabiensis and culicine mosquitoes in rice fields. Acta Trop. 2004 Jan;89(2):187-92. [Content Brief]
[6]. SASTRY KS, et al. The nature of the effect of ammonium sulphate on the biosynthesis of ascorbic acid in plants. Biochem J. 1956 Mar;62(3):451-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 7.5677 mL | 37.8387 mL | 75.6773 mL | 189.1933 mL |
| 5 mM | 1.5135 mL | 7.5677 mL | 15.1355 mL | 37.8387 mL | |
| 10 mM | 0.7568 mL | 3.7839 mL | 7.5677 mL | 18.9193 mL | |
| 15 mM | 0.5045 mL | 2.5226 mL | 5.0452 mL | 12.6129 mL | |
| 20 mM | 0.3784 mL | 1.8919 mL | 3.7839 mL | 9.4597 mL | |
| 25 mM | 0.3027 mL | 1.5135 mL | 3.0271 mL | 7.5677 mL | |
| 30 mM | 0.2523 mL | 1.2613 mL | 2.5226 mL | 6.3064 mL | |
| 40 mM | 0.1892 mL | 0.9460 mL | 1.8919 mL | 4.7298 mL | |
| 50 mM | 0.1514 mL | 0.7568 mL | 1.5135 mL | 3.7839 mL | |
| 60 mM | 0.1261 mL | 0.6306 mL | 1.2613 mL | 3.1532 mL | |
| 80 mM | 0.0946 mL | 0.4730 mL | 0.9460 mL | 2.3649 mL | |
| 100 mM | 0.0757 mL | 0.3784 mL | 0.7568 mL | 1.8919 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.