2. Cell Cycle/DNA Damage Cytoskeleton Apoptosis
  3. Microtubule/Tubulin Apoptosis Caspase Bcl-2 Family
  4. SP-6-27

SP-6-27 is a tubulin depolymerizing agent that binds to the colchicine site of β-tubulin. SP-6-27 induces G2/M cell cycle arrest in ovarian cancer cells. SP-6-27 enhances intrinsic apoptosis in ovarian cancer cells through upregulation of Bax, Apaf-1, caspase-6, caspase-9, and caspase-3. SP-6-27 reduces ovarian cancer cell migration. SP-6-27 inhibits capillary tube formation by human umbilical vein endothelial cells. SP-6-27 shows minimum cytotoxicity to normal ovarian epithelia. SP-6-27 shows enhanced cytotoxicity in chemo-sensitive/resistant ovarian cancer cells when combined with Cisplatin (HY-17394). SP-6-27 can be used for the research of ovarian cancer.

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SP-6-27

SP-6-27 Chemical Structure

CAS No. : 1384170-58-4

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SP-6-27 Related Antibodies

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Description

SP-6-27 is a tubulin depolymerizing agent that binds to the colchicine site of β-tubulin. SP-6-27 induces G2/M cell cycle arrest in ovarian cancer cells. SP-6-27 enhances intrinsic apoptosis in ovarian cancer cells through upregulation of Bax, Apaf-1, caspase-6, caspase-9, and caspase-3. SP-6-27 reduces ovarian cancer cell migration. SP-6-27 inhibits capillary tube formation by human umbilical vein endothelial cells. SP-6-27 shows minimum cytotoxicity to normal ovarian epithelia. SP-6-27 shows enhanced cytotoxicity in chemo-sensitive/resistant ovarian cancer cells when combined with Cisplatin (HY-17394). SP-6-27 can be used for the research of ovarian cancer[1].

IC50 & Target

Bax

 

Caspase 3

 

Caspase-6

 

Caspase-9

 

In Vitro

SP-6-27 (0.001-10 μM; 72 h) potently inhibits proliferation in Cisplatin-sensitive and resistant ovarian cancer cells and exhibits minimal cytotoxicity to normal HOSEpiC cells (IC50 = 0.10-0.84 μM for OVCA cells, 83.35 μM for HOSEpiC cells)[1].
SP-6-27 (0.5 μM; 24 h) disrupts alpha-tubulin and beta-tubulin cytoskeletal structure, reducing microtubule density in ovarian cancer cells[1].
SP-6-27 (0.5 μM; 48 h) reduces migration in ovarian cancer cells (A2780)[1].
SP-6-27 (0.5 μM; 24 h) induces G2-M cell cycle arrest in Cisplatin-sensitive and resistant ovarian cancer cells[1].
SP-6-27 (0.5 μM; 24-48 h) induces apoptosis in Cisplatin-sensitive and resistant ovarian cancer cells[1].
SP-6-27 upregulates pro-apoptotic genes (Bax, PAF-1, caspase-9, caspase-6, Caspase-3) and GADD45A in ovarian cancer cells[1].
SP-6-27 (0.5 μM; 24-48 h) enhances cytotoxicity in Cisplatin-sensitive cells when treated simultaneously with Cisplatin and in Cisplatin-resistant cells when treated sequentially[1].
SP-6-27 (0.5-1 μM; 24 h) inhibits capillary tube formation by HUVECs, even in the presence of tumor conditioned media[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SP-6-27 Related Antibodies

Cell Viability Assay[1]

Cell Line: cisplatin-sensitive OVCA (A2780, SKOV-3, TOV-112D), cisplatin-resistant OVCA (OVCAR-3, cis-A2780, cis-TOV-112D), HOSEpiC
Concentration: 0.001-10 μM
Incubation Time: 72 h
Result: Exhibited potent cytotoxicity against Cisplatin-sensitive OVCA cells with a mean IC50 of 0.14 μM and against cisplatin-resistant OVCA cells with a mean IC50 of 0.36 μM; showed minimal cytotoxicity with an IC50 of 83.35 μM in normal HOSEpiC cells.

Cell Migration Assay [1]

Cell Line: A2780 OVCA cells
Concentration: 0.5 μM
Incubation Time: 48 h
Result: Reduced migration compared to control cells when monitored over 48 h.

Cell Cycle Analysis[1]

Cell Line: Cisplatin-sensitive A2780 and Cisplatin-resistant cis-A2780 OVCA cells
Concentration: 0.5 μM
Incubation Time: 24 h
Result: Caused a complete collapse of cells in G1 phase and a substantial increase in G2-M phase cells: 87.8% of A2780 cells were arrested in G2-M phase (vs. 16.40% in control) and 58.9% of cis-A2780 cells were arrested in G2-M phase (vs. 14.5% in control).

Apoptosis Analysis[1]

Cell Line: Cisplatin-sensitive A2780 and Cisplatin-resistant cis-A2780 OVCA cells
Concentration: 0.5 μM
Incubation Time: 24 h (caspase-3 microscopy); 24, 48 h (Annexin V/7-AAD)
Result: Caused a pronounced increase in Annexin-V positive cells (early/late apoptotic) in both cell types at 24 and 48 h; showed increased cleaved caspase-3 activity in A2780 cells compared to control cells.
Molecular Weight

384.48

Formula

C24H24N4O
CAS No.
SMILES

N#CC1=C(OC2=CC(=CC=C2C1C=3C=CC(=C4C=CC=CC43)N(C)C)N(C)C)N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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SP-6-27 Related Classifications

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Keywords:

SP-6-271384170-58-4Microtubule/TubulinApoptosisCaspaseBcl-2 Familycaspase-6intrinsic apoptosiscytoskeletal structurealpha-tubulincapillary tube formationApaf-1ovarian cancer cell migrationGADD45AHOSEpiC cellstumor conditioned mediahuman cisplatin sensitive and resistant ovarian cancer cell linescisplatin sensitive/resistant ovarian cancer cellsBaxcolchicine sitehuman umbilical vein endothelial cellstubulinHUVECscaspase-3normal ovarian epitheliaG2/M cell cycle arrestcisplatinbeta-tubulinpro-apoptotic genescaspase-9microtubule densityInhibitorinhibitorinhibit

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