Tetrahydropapaverine hydrochloride
Based on 1 Customer Validation
Tetrahydropapaverine hydrochloride is a tetrahydroisoquinoline (TIQ) compound with inhibitory effects on tryptophan hydroxylase (TPH) (IC50 = 5.7 μM). Tetrahydropapaverine hydrochloride decreases intracellular serotonin content in murine mastocytoma P815 cells, with an IC50 value of 6.2 μM. Tetrahydropapaverine hydrochloride can be used for studies on potential neurotoxicity in monoaminergic neurons.
For research use only. We do not sell to patients.
- Purity: 99.08%
- CAS No.: 6429-04-5
- Formula: C20H26ClNO4
- Molecular Weight:379.88
-
Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
HY-12347
Ro 61-8048
-
HY-13289A
Nepicastat hydrochloride
-
HY-13055B
Telotristat
-
HY-13055
Telotristat etiprate
-
HY-15893
DMOG
-
HY-15127
Isotretinoin
-
HY-10581
Gatifloxacin
-
HY-15893R
DMOG (Standard)
-
HY-15127R
Isotretinoin (Standard)
-
HY-B1836
Meldonium
-
HY-12347R
Ro 61-8048 (Standard)
-
HY-13055BR
Telotristat (Standard)
Biological Activity
Tetrahydropapaverine (2.5-10 μM, 24 h) hydrochloride concentration-dependently decreases intracellular serotonin content in P815 cells, with an IC50 value of 6.2 μM[1].
Tetrahydropapaverine (5-20 μM, 24 h) hydrochloride significantly inhibits intracellular tryptophan hydroxylase (TPH) activity in P815 cells with an IC50 value of 5.7 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:8-week-old male Sprague-Dawley rats (250–320 g)[2]
-
Dosage:10 mg/kg
-
Administration:intracerebral injection, 7 days
-
Result:Caused significant reduction of striatal dopamine on injected side.
Reduced tyrosine hydroxylase-positive nigral neurons.
Increased DOPAC/dopamine ratio.
Chemical Information
-
CAS No. 6429-04-5
-
Appearance Solid
-
Molecular Weight 379.88
-
Formula C20H26ClNO4
-
Color White to off-white
-
SMILES
COC1=CC2=C(C=C1OC)CCNC2CC3=CC=C(OC)C(OC)=C3.[H]Cl
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : ≥ 100 mg/mL (263.24 mM)
DMSO : 50 mg/mL (131.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
-
Handling Instructions (2659 KB)
References
[1]. Kim EI, et al. Inhibitory effects of tetrahydropapaverine on serotonin biosynthesis in murine mastocytoma P815 cells. Life Sci. 2004 Sep 3;75(16):1949-57. [Content Brief]
[2]. Koshimura I, et al. Dimethoxyphenylethylamine and tetrahydropapaverine are toxic to the nigrostriatal system. Brain Res. 1997 Oct 31;773(1-2):108-16. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.6324 mL | 13.1621 mL | 26.3241 mL | 65.8103 mL |
| 5 mM | 0.5265 mL | 2.6324 mL | 5.2648 mL | 13.1621 mL | |
| 10 mM | 0.2632 mL | 1.3162 mL | 2.6324 mL | 6.5810 mL | |
| 15 mM | 0.1755 mL | 0.8775 mL | 1.7549 mL | 4.3874 mL | |
| 20 mM | 0.1316 mL | 0.6581 mL | 1.3162 mL | 3.2905 mL | |
| 25 mM | 0.1053 mL | 0.5265 mL | 1.0530 mL | 2.6324 mL | |
| 30 mM | 0.0877 mL | 0.4387 mL | 0.8775 mL | 2.1937 mL | |
| 40 mM | 0.0658 mL | 0.3291 mL | 0.6581 mL | 1.6453 mL | |
| 50 mM | 0.0526 mL | 0.2632 mL | 0.5265 mL | 1.3162 mL | |
| 60 mM | 0.0439 mL | 0.2194 mL | 0.4387 mL | 1.0968 mL | |
| 80 mM | 0.0329 mL | 0.1645 mL | 0.3291 mL | 0.8226 mL | |
| 100 mM | 0.0263 mL | 0.1316 mL | 0.2632 mL | 0.6581 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Tetrahydropapaverine hydrochloride Related Classifications
HY-N0137 Related Classifications
Powered by Bioz